IPP-1, 1-171aa, Human, His-tagged, Recombinant, E.coli

Protein phosphatase inhibitor-1(IPP-1) plays an important role in the regulation of glycogen metabolism through inhibition of type-1 protein serine/threonine phosphatase(PP1) activity, and it has been implicated in the regulation of cell growth. IPP-1 activation may impose cAMP control over proteins that are not directly phosphorylated by PKA. In the presence of calcium, PPI-1 is inactivated by calcineurin (or PP2B). Recombinant human IPP-1, fused to His-tag at C-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

1 mg/ml (determined by Bradford assay)

• Weiser DC., et al. (2004), J. Biol. Chem. 279(47):48904-14
• Rodriguez P., et al. (2006), J. Biol. Chem. 281(50):38599-608

>, 90% by SDS - PAGE

MEQDNSPRKI QFTVPLLEPH LDPEAAEQIR RRRPTPATLV LTSDQSSPEI DEDRIPNPHL KSTLAMSPRQ RKKMTRITPT MKELQMMVEH HLGQQQQGEE PEGAAESTGT QESRPPGIPD TEVESRLGTS GTAKKTAECI PKTHERGSKE PSTKEPSTHI PPLDSKGANS VLEHHHHHH