Comparison

Beta-lactamase, 20-377aa, E.coli, His tag, E.coli

Item no. ARP-01-P0942-0.5
Manufacturer American Research Products
Amount 0.5 mg
Quantity options 0.5 mg 100 ug 20 ug
Category
Type Proteins Recombinant
Specific against other
Purity > 95% by SDS - PAGE
Sequence MGSSHHHHHH SSGLVPRGSH MAPQQINDIV HRTITPLIEQ QKIPGMAVAV IYQGKPYYFT WGYADIAKKQ PVTQQTLFEL GSVSKTFTGV LGGDAIARGE IKLSDPTTKY WPELTAKQWN GITLLHLATY TAGGLPLQVP DEVKSSSDLL RFYQNWQPAW APGTQRLYAN SSIGLFGALA VKPSGLSFEQ AMQTRVFQPL KLNHTWINVP PAEEKNYAWG YREGKAVHVS PG
Citations Jaurin B., et al. (1981) Proc. Natl. Acad. Sci. U.S.A. 78:4897-4901Morandi F, et al. (2003) J AM Chem Soc. 125(3):685-95
NCBI NP_418574
ECLASS 10.1 32160409
ECLASS 11.0 32160409
UNSPSC 12352202
Alias ampC, ampA, Cephalosporinase, D-alanine carboxypeptidase
Available
Description
AmpC, also known as Beta-lactamase, is the most widespread resistance mechanism to beta-lactam antibiotics, such as the penicillins and the cephalosporins. These antibiotics have a common element in their molecular structure: a four-atom ring known as a beta-lactam. The lactamase enzyme breaks that ring open, deactivating the molecule's antibacterial properties. Recombinant E.coli beta-lactamase protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
Form
Liquid. 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol
Storage
Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
Synonyms
ampC, ampA, Cephalosporinase, D-alanine carboxypeptidase
Concentration
1 mg/ml (determined by Bradford assay)
NCBI
NP_418574
References
  • Jaurin B., et al. (1981) Proc. Natl. Acad. Sci. U.S.A. 78:4897-4901
  • Morandi F, et al. (2003) J AM Chem Soc. 125(3):685-95
Antigen Recognized in Species
E.coli
Purity
>, 95% by SDS - PAGE
Molecular Weight
41.8 kDa (379aa) confirmed by MALDI-TOF
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MAPQQINDIV HRTITPLIEQ QKIPGMAVAV IYQGKPYYFT WGYADIAKKQ PVTQQTLFEL GSVSKTFTGV LGGDAIARGE IKLSDPTTKY WPELTAKQWN GITLLHLATY TAGGLPLQVP DEVKSSSDLL RFYQNWQPAW APGTQRLYAN SSIGLFGALA VKPSGLSFEQ AMQTRVFQPL KLNHTWINVP PAEEKNYAWG YREGKAVHVS PGALDAEAYG VKSTIEDMAR WVQSNLKPLD INEKTLQQGI QLAQSRYWQT GDMYQGLGWE MLDWPVNPDS IINGSDNKIA LAARPVKAIT PPTPAVRASW VHKTGATGGF GSYVAFIPEK ELGIVMLANK NYPNPARVDA AWQILNALQ
Gene Category
Metabolism

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 0.5 mg
Available: In stock
available

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