RDH12, 39-316aa, Human, His tag, E.coli

Retinol dehydrogenase 12, also known as RDH12, belongs to the short-chain dehydrogenases /reductases (SDR) family. RDH12 is expressed, mostly in eye, kidney, brain, skeletal muscle and stomach. It is an NADPH-dependent retinal reductase whose highest activity is toward 9-cis and all-trans-retinol. RDH12 also plays a role in the metabolism of short-chain aldehydes but does not exhibit steroid dehydrogenase activity. Recombinant human RDH12 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

0.25 mg/ml (determined by Bradford assay)

• Maeda A., et al. (2006) J Biol Chem. 281:37697-37704.
• Keller B., et al. (2007) J Steroid Biochem Mol Biol. 104:190-194.

>, 90% by SDS - PAGE

MGSSHHHHHH SSGLVPRGSH MGSHMGKVVV ITGANTGIGK ETARELASRG ARVYIACRDV LKGESAASEI RVDTKNSQVL VRKLDLSDTK SIRAFAEGFL AEEKQLHILI NNAGVMMCPY SKTADGFETH LGVNHLGHFL LTYLLLERLK VSAPARVVNV SSVAHHIGKI PFHDLQSEKR YSRGFAYCHS KLANVLFTRE LAKRLQGTGV TTYAVHPGVV RSELVRHSSL LCLLWRLFSP FVKTAREGAQ TSLHCALAEG LEPLSGKYFS DCKRTWVSPR ARNNKTAERL WNVSCELLGI RWE