CLTA, 1-218aa , Human, His tag, E.coli

Clathrin, light chain A, also known as CLTA, belongs to the clathrin family. Clathrin is a large, soluble protein composed of heavy and light chains. It is a major cytosolic coat protein in pits and vesicles originating from the plasma membrane and the trans-Golgi network. In receptor-mediated endocytosis, receptor proteins are captured by Clathrin-coated vesicles. CLTA is one of two clathrin light chain proteins which are believed to function as regulatory elements. Recombinant human CLTA protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

1 mg/ml (determined by Bradford assay)

• Kirchhausen T., et al. (1987) Proc Natl Acad Sci USA. 84:8805-8809.
• Liu S H., et al. (1995) Cell. 83: 257-267.

>, 90% by SDS - PAGE

MGSSHHHHHH SSGLVPRGSH MGSHMAELDP FGAPAGAPGG PALGNGVAGA GEEDPAAAFL AQQESEIAGI ENDEAFAILD GGAPGPQPHG EPPGGPDAVD GVMNGEYYQE SNGPTDSYAA ISQVDRLQSE PESIRKWREE QMERLEALDA NSRKQEAEWK EKAIKELEEW YARQDEQLQK TKANNRAAEE AFVNDIDESS PGTEWERVAR LCDFNPKSSK QAKDVSRMRS VLISLKQAPL VH