MUTED, 1-187aa, Human, His tag, E.coli

Muted homolog, also known as MUTED, is involved in the development of lysosome related organelles, such as melanosomes and platelet dense granules. MUTED interacts with pallidin, dystrobrevin binding protein 1 and CNO/cappuccino. It is part of the biogenesis of lysosome related organelles complex 1 (BLOC1). Also, MUTED is ubiquitously expressed with higher levels in brain, bone marrow, kidney, and liver and lower levels in skeletal muscle. Recombinant human MUTED protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

0.25 mg/ml (determined by Bradford assay)

• Ciciotte S L., et al. (2003) Blood. 101: 4402-4407
• Starcevic M., et al. (2004) J Biol Chem. 279: 28393-28401.

>, 90% by SDS - PAGE

MGSSHHHHHH SSGLVPRGSH MGSMSGGGTE TPVGCEAAPG GGSKKRDSLG TAGSAHLIIK DLGEIHSRLL DHRPVIQGET RYFVKEFEEK RGLREMRVLE NLKNMIHETN EHTLPKCRDT MRDSLSQVLQ RLQAANDSVC RLQQREQERK KIHSDHLVAS EKQHMLQWDN FMKEQPNKRA EVDEEHRKAM ERLKEQYAEM EKDLAKFSTF