NDUFA2, 1-99 aa, Human, His tag, E.coli

NDUFA2 is a subunit of the hydrophobic protein fraction of the NADH:ubiquinone oxidoreductase (complex 1), the first enzyme complex in the electron transport chain located in the inner mitochondrial membrane, and may be involved in regulating complex I activity or its assembly via assistance in redox. Mutations in this protein are associated with Leigh syndrome, an early-onset progressive neurodegenerative disorder. Recombinant human NDUFA2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

1 mg/ml (determined by Bradford assay)

• Murray, J., et al. (2003) J. Biol. Chem. 278 (16), 13619-13622
• Loeffen, J.L.., et al. (1998) Biochem. Biophys. Res. Commun. 253 (2), 415-422.

>, 95% by SDS - PAGE

MGSSHHHHHH SSGLVPRGSH MGSMAAAAAS RGVGAKLGLR EIRIHLCQRS PGSQGVRDFI EKRYVELKKA NPDLPILIRE CSDVQPKLWA RYAFGQETNV PLNNFSADQV TRALENVLSG KA