PIP4K2B, 1-416aa Human, His tag, E.coli

PIP4K2B catalyzes the phosphorylation of phosphatidylinositol-4-phosphate on the fifth hydroxyl of the myo-inositol ring to form phosphatidylinositol-4, 5-bisphosphate. It is a member of the phosphatidylinositol-4-phosphate 5-kinase family. The amino acid sequence of PIP4K2B does not show similarity to other kinases, but the protein does exhibit kinase activity. Additionally, PIP4K2B interacts with p55 TNF receptor. Recombinant human PIP4K2B protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

0.5 mg/ml (determined by Bradford assay)

• Richardson JP, Wang M, et al. (2007). Cell Signal. 19(6):1309-14.
• Luoh SW, Venkatesan N, et al. (2004). Oncogene. 23(7):1354-63.

>, 90% by SDS - PAGE

MGSSHHHHHH SSGLVPRGSH MGSMSSNCTS TTAVAVAPLS ASKTKTKKKH FVCQKVKLFR ASEPILSVLM WGVNHTINEL SNVPVPVMLM PDDFKAYSKI KVDNHLFNKE NLPSRFKFKE YCPMVFRNLR ERFGIDDQDY QNSVTRSAPI NSDSQGRCGT RFLTTYDRRF VIKTVSSEDV AEMHNILKKY HQFIVECHGN TLLPQFLGMY RLTVDGVETY MVVTRNVFSH RLTVHRKYDL KGSTVAREAS DKEKAKDLPT FKDNDFLNEG QKLHVGEESK KNFLEKLKRD VEFLAQLKIM DYSLLVGIHD VDRAEQEEME VEERAEDEEC ENDGVGGNLL CSYGTPPDSP GNLLSFPRFF GPGEFDPSVD VYAMKSHESS PKKEVYFMAI IDILTPYDTK KKAAHAAKTV KHGAGAEIST VNPEQYSKRF NEFMSNILT