PMF1-BGLAP, 1-211aa, Human, His tag, E.coli

This locus represents naturally occurring read-through transcription between the neighboring PMF1 (polyamine-modulated factor 1) and BGLAP (bone gamma-carboxyglutamate Gla protein) genes on chromosome 1. Alternative splicing results in multiple transcript variants encoding isoforms that share sequence identity with the upstream gene product, but they contain distinct C-termini due to frameshifts versus the downstream gene coding sequence. Recombinant human PMF1-BGLAP protein, fused to His-tag at N-terminus, was expressed in E.coli.

Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

1mg/ml (determined by Bradford assay)

• Udeshi ND. et al. (2012) Mol Cell Proteomics. 11:148-159.
• Strausberg RL. et al. (2002) Proc Natl Acad Sci U S A. 99:16899-16903

>, 85% by SDS - PAGE

MGSSHHHHHH SSGLVPRGSH MGSMAEASSA NLGSGCEEKR HEGSSSESVP PGTTISRVKL LDTMVDTFLQ KLVAAGSYQR FTDCYKCFYQ LQPAMTQQIY DKFIAQLQTS IREEISDIKE EGNLEAVLNA LDKIVEEGKV RKEPAWRPSG IPEKDLHSVM APYFLQQRDT LRRHVQKQEA ENQQLADAVL AGRRQVEELQ LQVQAQQQAW QVRSPAVQSP AKVQPLCPSR RAAR