SSR1, 22-207aa, Human, His tag, E.coli

The signal sequence receptor (SSR) is a glycosylated endoplasmic reticulum (ER) membrane receptor associated with protein translocation across the ER membrane. The SSR consists of 2 subunits, a 34-kD glycoprotein encoded by this gene and a 22-kD glycoprotein. This gene generates several mRNA species as a result of complex alternative polyadenylation. This gene is unusual in that it utilizes arrays of polyA signal sequences that are mostly non-canonical. Recombinant human SSR1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

0.25 mg/ml (determined by Bradford assay)

• Hartmann E., Prehn S. (1994), FEBS Lett. 349:324-326
• Hirama T., Miller C.W., et al. (1999), FEBS Lett. 455:223-227

>, 90% by SDS - PAGE

MGSSHHHHHH SSGLVPRGSH MGSRGGPRGL LAVAQDLTED EETVEDSIIE DEDDEAEVEE DEPTDLVEDK EEEDVSGEPE ASPSADTTIL FVKGEDFPAN NIVKFLVGFT NKGTEDFIVE SLDASFRYPQ DYQFYIQNFT ALPLNTVVPP QRQATFEYSF IPAEPMGGRP FGLVINLNYK DLNGNVFQDA VFNQTVTVIE REDGLDGET