RPL31, 1-125aa, Human, His tag, E.coli

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L31E family of ribosomal proteins. It is located in the cytoplasm. Higher levels of expression of this gene in familial adenomatous polyps compared to matched normal tissues have been observed. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Recombinant human RPL31 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

0.25 mg/ml (determined by Bradford assay)

Ewing RM, Chu P, et al. (2007). Mol Syst Biol. 3:89.

>, 85% by SDS - PAGE

MGSSHHHHHH SSGLVPRGSH MGSMAPAKKG GEKKKGRSAI NEVVTREYTI NIHKRIHGVG FKKRAPRALK EIRKFAMKEM GTPDVRIDTR LNKAVWAKGI RNVPYRIRVR LSRKRNEDED SPNKLYTLVT YVPVTTFKNL QTVNVDEN