LGALS13, 1-139aa, Human, His tag, E.coli

Lysophospholipases are enzymes that act on biological membranes to regulate the multifunctional lysophospholipids. The protein encoded by this gene has lysophospholipase activity. It is composed of two identical subunits which are held together by disulfide bonds. This protein has structural similarity to several members of the beta-galactoside-binding S-type lectin family. Recombinant human LGALS13 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

0.25 mg/ml (determined by Bradford assay)

• Than NG, Pick E, et al. (2004). Eur J Biochem. 271(6):1065-78.
• Visegrady B, Than NG, et al. (2001). Protein Eng. 14(11):875-80.

>, 95% by SDS - PAGE

MGSSHHHHHH SSGLVPRGSH MGSMSSLPVP YKLPVSLSVG SCVIIKGTPI HSFINDPQLQ VDFYTDMDED SDIAFRFRVH FGNHVVMNRR EFGIWMLEET TDYVPFEDGK QFELCIYVHY NEYEIKVNGI RIYGFVHRIP PSFVKMVQVS RDISLTSVCV CN