RAB2A, 1-212aa, Human, His tag, E.coli

RAB2A(Ras-related protein Rab-2A) belongs to the small GTPase superfamily. Members of the RAB protein family are nontransforming monomeric GTP-binding proteins of the Ras superfamily that contain 4 highly conserved regions involved in GTP binding and hydrolysis. RAB proteins are also an integral part of endocytic pathways. RAB2A and RAB2B are required for protein transport from the ER to the Golgi, RAB2A is lipid-anchored to the ER-Golgi intermediate compartment membrane while RAB2B is lipid anchored to the cytoplasmic side of the cell membrane. RAB2A has been shown to interact with GOLGA2. Recombinant human RAB2A protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

0.5mg/ml (determined by Bradford assay)

• Opdam, F.J., et al. (2000) Eur. J. Cell Biol. 79: 308-316.
• Tisdale E.J. (2003) J. Biol. Chem. 278:52524-52530

>, 90% by SDS - PAGE

MGSSHHHHHH SSGLVPRGSH SGSHMAYAYL FKYIIIGDTG VGKSCLLLQF TDKRFQPVHD LTIGVEFGAR MITIDGKQIK LQIWDTAGQE SFRSITRSYY RGAAGALLVY DITRRDTFNH LTTWLEDARQ HSNSNMVIML IGNKSDLESR REVKKEEGEA FAREHGLIFM ETSAKTASNV EEAFINTAKE IYEKIQEGVF DINNEANGIK IGPQHAATNA THAGNQGGQQ AGGGCC