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Biotinylated Human B7-H3/CD276 Protein (BH7-HM473B)

Biotinylated Human B7-H3/CD276 Protein (BH7-HM473B)

Item no.	BH7-HM473B-500UG
Manufacturer	KactusBio
Amount	500 ug
Quantity options	100 ug 500 ug
Category	Oncology By Cell Type Carcinoma Melanoma Sarcoma Peptides & Proteins Recombinant Proteins Mesothelioma Blastom
Type	Proteins Recombinant
Format	Lyophilized
Specific against	Human (Homo sapiens)
Host	HEK293
Conjugate/Tag	HIS, Avi
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Formula	Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Sequence	Leu29-Pro245
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	B7H3,B7-H3,CD276,PSEC0249, UNQ309,PRO352,B7 homolog 3,CD276
Shipping Condition	Room temperature
Available
Manufacturer - Type	Recombinant Protein
Manufacturer - Targets	B7-H3/CD276
Manufacturer - Conjugate / Tag	C-His-Avi
Shipping Temperature	Shipped at ambient temperature.
Storage Conditions	-20 to -80°C for 24 months as supplied from date of receipt.； -80°C for 3 months after reconstitution.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Molecular Weight	The protein has a predicted MW of 26.49 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Bis-Tris PAGE result.
Description	Recombinant Biotinylated Human B7-H3/CD276 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Leu29-Pro245.
Background	B7-H3, a member of the B7 family of immunomodulatory molecules, is overexpressed in a wide range of solid cancers. B7-H3 binds to activated T cells via an as yet unidentified receptor. In assays using sub-optimal amount so anti-CD3 stimulation, 2Ig‑B7‑H3 enhances T cell proliferation, T cell interferon-gamma (IFN-gamma) production, and cytotoxic T cells induction.
Endotoxin	Less than 1 EU per μg by the LAL method.
Formulation	Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1mg/ml for 500μg or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Biotinylated	yes

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Request Data Sheet

BH7-HM473B-500UG-datasheet.pdf

BH7-HM473B-500UG-safety-datasheet.pdf