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Mouse BID Protein (BID-ME101)

Mouse BID Protein (BID-ME101)

Item no.	BID-ME101-500UG
Manufacturer	KactusBio
Amount	500 ug
Quantity options	100 ug 500 ug
Category	Peptides & Proteins Recombinant Proteins Cell Biology Cell Status Apoptosis Autophagy
Type	Proteins Recombinant
Format	Lyophilized
Specific against	Mouse (Murine, Mus musculus)
Host	E.coli
Conjugate/Tag	HIS
Purity	> 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Sequence	Ala1-Ser195
NCBI	EDK99650.1
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	p22 BID,BID
Shipping Condition	Room temperature
Available
Manufacturer - Type	Recombinant Protein
Manufacturer - Targets	BID
Manufacturer - Conjugate / Tag	C-His
Shipping Temperature	Shipped at ambient temperature.
Storage Conditions	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Molecular Weight	The protein has a predicted MW of 22.72 kDa same as Bis-Tris PAGE result.
Description	Recombinant Mouse BID Protein is expressed from E.coli with His tag at the C-Terminus.It contains Ala1-Ser195.
Background	Bid, BH3-interacting domain death agonist, was initially cloned based in its ability to interact with both Bcl-2 and Bax. Bid contains only the BH3 domain, which is required for its interaction with the Bcl-2 family proteins and for its pro-death activity. Bid is susceptible to proteolytic cleavage by caspases, calpains, Granzyme B and cathepsins. Bid is important to cell death mediated by these proteases and thus is the sentinel to protease-mediated death signals.
Endotoxin	Less than 1 EU per μg by the LAL method.
Formulation	Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1mg/ml for 500μg or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Biotinylated	no

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Request Data Sheet

BID-ME101-500UG-datasheet.pdf

BID-ME101-500UG-safety-datasheet.pdf