Comparison

Human Carbonic Anhydrase IX / CA9 (138-414) Protein, His Tag (MALS verified) European Partner

Item no. CA9-H5220-1mg
Manufacturer ACROBiosystems
Amount 1 mg
Quantity options 1 mg 50 ug
Category
Type Proteins Recombinant
Format Liquid
Specific against Human (Homo sapiens)
Host HEK293
Conjugate/Tag Unconjugated, HIS
Purity 95%
Dry ice Yes
Citations (1) Badger MR, Price GD, 1994, Annu. Rev. Plant Physiol. Plant Mol. Bio. 45: 369-392. (2) Temperini C., et al., 2007, Bioorg. Med. Chem. Lett. 17:2210-2215. (3) Hilvo M., et al., 2008, J. Biol. Chem. 283:27799-27809. (4) Alterio V., et al., 2009, Proc. Natl. Acad. Sci. U.S.A. 106:16233-16238.
NCBI NP_001207.2
ECLASS 10.1 32160409
ECLASS 11.0 32160409
UNSPSC 12352202
Alias CAIX,CA9,CA-IX,G250,MN,P54/58N,pMW1
Shipping Condition Dry ice
Available
Manufacturer - Category
Protein / Bio-Markers & CD Antigens
Manufacturer - Conjugate / Tag
C-6xHis, Unconjugated
Shipping Temperature
Dry ice
Storage Conditions
-70°C
Molecular Weight
30.9 kDa
Manufacturer - Format
Liquid
Description
Human Carbonic Anhydrase IX (138-414), His Tag (CA9-H5220) is expressed from human 293 cells (HEK293). It contains AA Gln 138 - Asp 414 (Accession # NP_001207.2).
Background
Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes. CAs form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons (or vice versa), a reversible reaction that occurs rather slowly in the absence of a catalyst. One of the functions of the enzyme in animals is to interconvert carbon dioxide and bicarbonate to maintain acid-base balance in blood and other tissues, and to help transport carbon dioxide out of tissues. The active site of most carbonic anhydrases contains a zinc ion. There are at least five distinct CA families (α, β, γ, δ and ε).
Carbonic anhydrase 9 (CA9 / CAIX) is also known as Membrane antigen MN (MN), Renal cell carcinoma-associated antigen G250, which belongs to the alpha-carbonic anhydrase family. CA9 / CAIX with an optimal activity at pH 6.49. Reversible hydration of carbon dioxide. CA IX participates in pH regulation. CA9 may be involved in the control of cell proliferation and transformation. CA-IX appears to be a novel specific biomarker for a cervical neoplasia.
Molecule
Carbonic Anhydrase IX
Exp Region
Gln 138 - Asp 414
Characteristics
This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 30.9 kDa. The protein migrates as 34 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
1.0 EU per μg
Buffer
50 mM Tris, 150 mM NaCl, pH7.5
Stability
● The product MUST be stored at -70°C or lower upon receipt;
● -70°C for 3 months under sterile conditions.
Protectant
trehalose

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 1 mg
Available: In stock
available

Compare

Add to wishlist

Get an offer

Request delivery time

Ask a technical question

Submit a bulk request

Questions about this Product?