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Human CALCA/CGRP Protein (CAL-HM201)

Human CALCA/CGRP Protein (CAL-HM201)

Item no.	CAL-HM201-500UG
Manufacturer	KactusBio
Amount	500 ug
Quantity options	100 ug 500 ug
Category	Oncology Neuroscience Molecular Targets for Neuroscience Peptides & Proteins Recombinant Proteins Cell Biology Cell Status Necrosis By Organ Thyroid Cancer
Type	Proteins Recombinant
Format	Lyophilized
Specific against	Human (Homo sapiens)
Host	HEK293
Conjugate/Tag	C-hFc
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Sequence	Ala26-Asn141
NCBI	NP_001732.1
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	Calcitonin,CCP,PDN-21,CALCA,CALC1,CGRP,CGRP-I,CGRP1,CT,KC,PCT,Procalcitonin,Calcitonin 1,Katacalcin
Shipping Condition	Room temperature
Available
Manufacturer - Type	Recombinant Protein
Manufacturer - Targets	CALCA/CGRP
Shipping Temperature	Shipped at ambient temperature.
Storage Conditions	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Molecular Weight	The protein has a predicted MW of 39.4 kDa. Due to glycosylation, the protein migrates to 45-48 kDa based on Bis-Tris PAGE result.
Description	Recombinant Human CALCA/CGRP Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Ala26-Asn141.
Background	Specific fragments of methylation changes in the target gene (Calca) were identified by IGV analysis. CGRP was applied to compare the effects on ASCs-T2DM morphology via phalloidin staining, proliferation through CCK-8 assay, and osteogenic differentiation with osteogenic staining, qPCR, and repair of calvarial defect. Furthermore, 5-azacytidine (5-az) was used to intervene ASCs-T2DM to verify the relationship between the methylation level of the target fragment and expression of Calca.
Endotoxin	Less than 1 EU per μg by the LAL method.
Formulation	Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1mg/ml for 500μg or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Biotinylated	no

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Request Data Sheet

CAL-HM201-500UG-datasheet.pdf

CAL-HM201-500UG-safety-datasheet.pdf