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Human CD73 Protein, Mouse IgG2a Fc Tag (active enzyme)

Human CD73 Protein, Mouse IgG2a Fc Tag (active enzyme)

Item no.	CD3-H5252-100ug
Manufacturer	ACROBiosystems
Amount	100 ug
Quantity options	100 ug 500 ug x 2
Category	Peptides & Proteins Recombinant Proteins
Type	Proteins Recombinant
Format	Solid
Specific against	Human (Homo sapiens)
Host	HEK293
Isotype	IgG2a
Conjugate/Tag	Unconjugated, C-mFc
Purity	95%
Citations	(1)Misumi Y., et al., 1990, Eur. J. Biochem., 191 (3): 563-569. (2)St. Hilaire, Cynthia, et al., 2011, New England Journal of Medicine, 364 (5): 432-442. (3)Sharp, J., 1954, Ann. Rheum. Dis., 13 (1): 15-27.
NCBI	NP_002517.1
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	CD73,NT5E,5'-Nucleotidase,5'-NT,NT5,NTE
Shipping Condition	Room temperature
Available
Manufacturer - Category	Protein
Manufacturer - Conjugate / Tag	C-mFc(mIgG2a), Unconjugated
Shipping Temperature	RT
Storage Conditions	-20°C
Molecular Weight	84.9 kDa
Manufacturer - Format	Powder
Description	5'-nucleotidase (5'-NT), also known as ecto-5'-nucleotidase or CD73 (cluster of differentiation 73), is an enzyme that is encoded by the NT5E gene. CD73 commonly serves to convert AMP to adenosine. Ecto-5-prime-nucleotidase (5-prime-ribonucleotide phosphohydrolase) catalyzes the conversion at neutral pH of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP. Other forms of 5-prime nucleotidase exist in the cytoplasm and lysosomes and can be distinguished from ecto-NT5 by their substrate affinities, requirement for divalent magnesium ion, activation by ATP, and inhibition by inorganic phosphate. Rare allelic variants are associated with a syndrome of adult-onset calcification of joints and arteries (CALJA) affecting the iliac, femoral, and tibial arteries reducing circulation in the legs and the joints of the hands and feet causing pain.
Background	5'-nucleotidase (5'-NT), also known as ecto-5'-nucleotidase or CD73 (cluster of differentiation 73), is an enzyme that is encoded by the NT5E gene. CD73 commonly serves to convert AMP to adenosine. Ecto-5-prime-nucleotidase (5-prime-ribonucleotide phosphohydrolase) catalyzes the conversion at neutral pH of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP. Other forms of 5-prime nucleotidase exist in the cytoplasm and lysosomes and can be distinguished from ecto-NT5 by their substrate affinities, requirement for divalent magnesium ion, activation by ATP, and inhibition by inorganic phosphate. Rare allelic variants are associated with a syndrome of adult-onset calcification of joints and arteries (CALJA) affecting the iliac, femoral, and tibial arteries reducing circulation in the legs and the joints of the hands and feet causing pain.
Molecule	CD73
Exp Region	Trp 27 - Ser 549
Characteristics	This protein carries a mouse IgG2a Fc tag at the C-terminus. The protein has a calculated MW of 84.9 kDa. The protein migrates as 95-100 kDa under reducing (R) condition (SDS-PAGE) due to Glycosylation.
Endotoxin	0.1 EU per μg
Buffer	50 mM Tris, 100 mM Glycine, 150 mM NaCl, pH7.5
Stability	● -20°C to -70°C for 12 months in lyophilized state ● -70°C for 3 months under sterile conditions after reconstitution._x000D_For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Protectant	trehalose

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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CD3-H5252-100ug-datasheet.pdf

CD3-H5252-100ug-safety-datasheet.pdf