Comparison

Biotinylated Human / Cynomolgus / Rhesus macaque CD28 Protein, Fc,Avitag™, active dimer (MALS verified) European Partner

Item no. CD8-H82F2-200ug
Manufacturer ACROBiosystems
Amount 200 ug
Quantity options 200 ug 25 ug
Category
Type Proteins Recombinant
Format Solid
Specific against Human (Homo sapiens), Monkey (Cynomolgus, Simian), Rhesus macaque
Host HEK293
Conjugate/Tag Biotin, Avi, Fc
Purity 95%
Citations (1)Evans E.J., et al., 2005, Nat. Immunol. 6:271-279. (2) Mayya V., et al., 2009, Sci. Signal. 2:RA46-RA46. (3) Wollscheid B., et al., 2009, Nat. Biotechnol. 27:378-386.
NCBI AAH93698
ECLASS 10.1 32160409
ECLASS 11.0 32160409
UNSPSC 12352202
Alias CD28,Tp44
Shipping Condition Room temperature
Available
Specificity Human / Cynomolgus / Rhesus macaque
Manufacturer - Category
Protein
Manufacturer - Conjugate / Tag
C-Fc & C-Avi, Biotin-labeled
Shipping Temperature
RT
Storage Conditions
-20°C
Molecular Weight
43.6 kDa
Manufacturer - Format
Powder
Description
Biotinylated Human / Cynomolgus / Rhesus macaque CD28, Fc, Avitag (CD8-H82F2) is expressed from human 293 cells (HEK293). It contains AA Asn 19 - Pro 152 (Accession # AAH93698). In the region Asn 19 - Pro 152, the AA sequence of Human, Cynomolgus and Rhesus macaque CD28 are homologus.
Background
T-cell-specific surface glycoprotein CD28 is also known as TP44, is a single-pass type I membrane protein which contains one Ig-like V-type (immunoglobulin-like) domain. is one of the molecules expressed on T cells that provide co-stimulatory signals, which are required for T cell activation. CD28 is the receptor for CD80 (B7.1) and CD86 (B7.2). When activated by Toll-like receptor ligands, the CD80 expression is upregulated in antigen presenting cells (APCs). The CD86 expression on antigen presenting cells is constitutive. CD28 is the only B7 receptor constitutively expressed on naive T cells.
Molecule
CD28
Exp Region
Asn 19 - Pro 152
Characteristics
This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™). The protein has a calculated MW of 43.6 kDa. The protein migrates as 55-70 kDa under reducing (R) condition, and 110-130 kDa under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.
Endotoxin
1.0 EU per μg
Buffer
Tris with Glycine, Arginine and NaCl, pH7.5
Stability
● -20°C to -70°C for 12 months in lyophilized state
● -70°C for 3 months under sterile conditions after reconstitution._x000D_For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Protectant
trehalose

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 200 ug
Available: In stock
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