Comparison

Biotinylated Human CD8 alpha / CD8A Protein, His,Avitag™ European Partner

Item no. CDA-H82E3-25ug
Manufacturer ACROBiosystems
Amount 25 ug
Quantity options 200 ug 25 ug
Category
Type Proteins Recombinant
Format Solid
Specific against Human (Homo sapiens)
Host HEK293
Conjugate/Tag Biotin, HIS, Avi
Purity 90%
Citations (1) Zhu J, et al. Nature. 2013. 497(7450):494-7. (2) Gibbings DJ, et al. BMC Immunol. 2007. 8:12. (3) Addison EG, et al. Immunology. 2005. 116(3):354-61.
NCBI NP_001759.3
ECLASS 10.1 32160409
ECLASS 11.0 32160409
UNSPSC 12352202
Alias CD8A,CD8,Leu2,MAL,p32
Shipping Condition Room temperature
Available
Manufacturer - Category
Protein / MABSol® Biotin Labeled Proteins
Manufacturer - Conjugate / Tag
C-10xHis & C-Avi, Biotin-labeled
Shipping Temperature
RT
Storage Conditions
-20°C
Molecular Weight
21.2 kDa
Manufacturer - Format
Powder
Description
Biotinylated Human CD8 alpha, His, Avitag (CDA-H82E3) is expressed from human 293 cells (HEK293). It contains AA Ser 22 - Asp 182 (Accession # P01732-1).
Background
Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class I proteins presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. In NK-cells, the presence of CD8A homodimers at the cell surface provides a survival mechanism allowing conjugation and lysis of multiple target cells. CD8A homodimer molecules also promote the survival and differentiation of activated lymphocytes into memory CD8 T-cells.
Molecule
CD8 alpha
Exp Region
Ser 22 - Asp 182
Characteristics
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™). The protein has a calculated MW of 21.2 kDa. The protein migrates as 25-32 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
0.1 EU per μg
Buffer
PBS, pH7.4
Stability
● -20°C to -70°C for 12 months in lyophilized state;
● -70°C for 3 months under sterile conditions after reconstitution._x000D_For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Protectant
trehalose

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 25 ug
Available: In stock
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