« Back
Home /
Biotinylated Human CD3E&CD3D Heterodimer Protein, Fc,His Tag&Fc,Flag Tag, ultra sensitivity (primary amine labeling) (MALS verified)

Biotinylated Human CD3E&CD3D Heterodimer Protein, Fc,His Tag&Fc,Flag Tag, ultra sensitivity (primary amine labeling) (MALS verified)

Item no.	CDD-H82W0-25ug
Manufacturer	ACROBiosystems
Amount	25 ug
Quantity options	200 ug 25 ug
Category	Peptides & Proteins Recombinant Proteins
Type	Proteins Recombinant
Format	Solid
Specific against	Human (Homo sapiens)
Host	HEK293
Conjugate/Tag	Biotin, FLAG, Fc
Purity	95%
Citations	(1) Gold DP, et al., 1986, Nature 321 (6068): 431-4. (2) Clevers HC, et al., 1988, Proc. Natl. Acad. Sci. U.S.A. 85 (21): 8156-60. (3) Gil J, et al., 2011, J Clin Invest, 121(10):3872-6. (4) Lu X, et al.,2011, Hematology, 16(3):185-9.
NCBI	NP_000724.1 (CD3E), NP_000723.1 (CD3D)
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	CD3E & CD3D,CD3 delta & CD3 epsilon,CD3 delta / epsilon
Shipping Condition	Room temperature
Available
Manufacturer - Category	Protein
Manufacturer - Conjugate / Tag	C-Fc & C-6xHis \| C-Fc & C-Flag, Biotin-labeled
Shipping Temperature	RT
Storage Conditions	-20°C
Molecular Weight	39.2 kDa (CD3E), 37.5 kDa (CD3D)
Manufacturer - Format	Powder
Description	MABSol® Biotinylated Human CD3E&CD3D Heterodimer Protein, Fc, His Tag&Fc, Flag Tag, primary amine labeling (CDD-H82W0) is expressed from human HEK293 cells. It contains AA Asp 23 - Asp 126 (CD3E) & Phe 22 - Ala 105 (CD3D) (Accession # NP_000724.1 (CD3E) & NP_000723.1 (CD3D)).
Background	T-cell surface glycoprotein CD3 delta & CD3 epsilon chain, also known as CD3D & CD3E or CD3D&CD3E respectively, are single-pass type I membrane proteins. CD3D, together with CD3- epsilon(CD3E) , CD3-gamma and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways.
Molecule	CD3E & CD3D
Exp Region	Asp 23 - Asp 126 (CD3E) & Phe 22 - Ala 105 (CD3D)
Characteristics	The subunit CD3E carries a human IgG1 Fc fragment at the C-terminus, followed by a polyhistidine tag. The subunit CD3D carries a human IgG1 Fc fragment at the C-terminus, followed by a flag tag. The protein has a calculated MW of 39.2 kDa (CD3E) and 37.5 kDa (CD3D) . The protein migrates as 45-55 kDa on a SDS-PAGE gel when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition due to glycosylation.
Endotoxin	1.0 EU per μg
Buffer	PBS, pH7.4
Stability	● -20°C to -70°C for 12 months in lyophilized state ● -70°C for 3 months under sterile conditions after reconstitution._x000D_For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Protectant	trehalose

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Request Data Sheet

CDD-H82W0-25ug-datasheet.pdf

CDD-H82W0-25ug-safety-datasheet.pdf