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Mouse CD3 epsilon&CD3 delta Heterodimer Protein, Fc,His Tag&Fc,Flag Tag (MALS verified)

Mouse CD3 epsilon&CD3 delta Heterodimer Protein, Fc,His Tag&Fc,Flag Tag (MALS verified)

Item no.	CDD-M52W2-100ug
Manufacturer	ACROBiosystems
Amount	100 ug
Quantity options	100 ug 500 ug x 2
Category	Peptides & Proteins Recombinant Proteins
Type	Proteins Recombinant
Format	Solid
Specific against	Mouse (Murine, Mus musculus)
Host	HEK293
Conjugate/Tag	Unconjugated, FLAG, Fc
Purity	95%
Citations	(1) Gold DP, et al., 1986, Nature 321 (6068): 431-4. (2) Clevers HC, et al., 1988, Proc. Natl. Acad. Sci. U.S.A. 85 (21): 8156-60. (3) Gil J, et al., 2011, J Clin Invest, 121(10):3872-6. (4) Lu X, et al.,2011, Hematology, 16(3):185-9.
NCBI	NP_031674.1 (CD3E), NP_038515.3 (CD3D)
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	CD3E & CD3D,CD3 delta & CD3 epsilon,CD3 delta / epsilon
Shipping Condition	Room temperature
Available
Manufacturer - Category	Protein
Manufacturer - Conjugate / Tag	C-Fc & C-10xHis \| C-Fc & C-Flag, Unconjugated
Shipping Temperature	RT
Storage Conditions	-20°C
Molecular Weight	41.5 kDa (CD3E) & 40.7 kDa (CD3D)
Manufacturer - Format	Powder
Description	T-cell surface glycoprotein CD3 delta & CD3 epsilon chain, also known as CD3D & CD3E or CD3D&CD3E respectively, are single-pass type I membrane proteins. CD3D, together with CD3- epsilon(CD3E) , CD3-gamma and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways.
Background	T-cell surface glycoprotein CD3 delta & CD3 epsilon chain, also known as CD3D & CD3E or CD3D&CD3E respectively, are single-pass type I membrane proteins. CD3D, together with CD3- epsilon(CD3E) , CD3-gamma and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways.
Molecule	CD3E & CD3D
Exp Region	Asp 23 - Asp 108 (CD3E) & Phe 22 - Ala 105 (CD3D)
Characteristics	Mouse CD3E&CD3D Heterodimer Protein, Fc, His Tag&Fc, Flag Tag is produced by co-expression of CD3E and CD3D, has a calculated MW of 41.5 kDa (CD3E) and 40.7 kDa (CD3D). Subunit CD3E is fused with a human IgG1 Fc tag and a polyhistidine tag at the C-terminus and subunit CD3D is fused with a human IgG1 Fc tag and a flag tag at the C-terminus. As a result of glycosylation, the heterodimer protein migrates as 55-65 kDa under reducing (R) condition, and 90-116 kDa under non-reducing (NR) condition (SDS-PAGE).
Endotoxin	1.0 EU per μg
Buffer	50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5
Stability	● -20°C to -70°C for 12 months in lyophilized state ● -70°C for 3 months under sterile conditions after reconstitution._x000D_For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Protectant	trehalose

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Request Data Sheet

CDD-M52W2-100ug-datasheet.pdf

CDD-M52W2-100ug-safety-datasheet.pdf