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Biotinylated Human CD3 epsilon&CD3 gamma Heterodimer Protein, His,Avitag™&Tag Free (MALS verified)

Biotinylated Human CD3 epsilon&CD3 gamma Heterodimer Protein, His,Avitag™&Tag Free (MALS verified)

Item no.	CDG-H82W3-200ug
Manufacturer	ACROBiosystems
Amount	200 ug
Quantity options	1 mg 200 ug 25 ug 50 ug
Category	Peptides & Proteins Recombinant Proteins
Type	Proteins Recombinant
Format	Solid
Specific against	Human (Homo sapiens)
Host	HEK293
Conjugate/Tag	Biotin, HIS, Avi
Purity	90%
NCBI	NP_000724.1 (CD3E) , AAI13831 (CD3G)
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	CD3 epsilon & CD3 gamma,CD3E & CD3G
Shipping Condition	Room temperature
Available
Manufacturer - Category	Protein / MABSol® Biotin Labeled Proteins
Manufacturer - Conjugate / Tag	C-10xHis & C-Avi \| Native, Biotin-labeled
Shipping Temperature	RT
Storage Conditions	-20°C
Molecular Weight	18.6 kDa & 14.7 kDa
Manufacturer - Format	Powder
Description	Biotinylated Human CD3E&CD3G Heterodimer Protein, His, Avitag&Tag Free (CDG-H82W3) is expressed from human 293 cells (HEK293). It contains AA Asp 23 - Asp 126 (CD3E) & Gln 23 - Ser 116 (CD3G) (Accession # NP_000724.1 (CD3E) & AAI13831 (CD3G)).
Background	T-cell surface glycoprotein CD3 delta & CD3 gamma chain, also known as CD3D & CD3G or CD3D&CD3G respectively, are single-pass type I membrane proteins. CD3D, together with CD3- epsilon(CD3E), CD3-gamma and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways.
Molecule	CD3E & CD3G
Exp Region	Asp 23 - Asp 126 (CD3E) & Gln 23 - Ser 116 (CD3G)
Characteristics	Biotinylated Human CD3E&CD3G Heterodimer Protein, His, Avitag&Tag Free is produced by co-expression of CD3E and CD3G, has a calculated MW of 18.6 kDa (CD3E) and 14.7 kDa (CD3G). Subunit CD3E is fused with a polyhistidine tag at the C-terminus, followed by an Avi tag and subunit CD3G contains no tag at the C-terminus. The reducing (R) heterodimer protein migrates as 20-24 kDa and 25-28 kDa when calibrated against Star Ribbon Pre-stained Protein Marker due to glycosylation.
Endotoxin	1.0 EU per μg
Buffer	PBS, pH 7.4
Stability	● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution._x000D_For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Protectant	trehalose

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Request Data Sheet

CDG-H82W3-200ug-datasheet.pdf

CDG-H82W3-200ug-safety-datasheet.pdf