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Human DKK1 Protein (DKK-HM401)

Human DKK1 Protein (DKK-HM401)

Item no.	DKK-HM401-500UG
Manufacturer	KactusBio
Amount	500 ug
Quantity options	100 ug 500 ug
Category	Oncology By Cell Type Carcinoma Peptides & Proteins Recombinant Proteins
Type	Proteins Recombinant
Format	Lyophilized
Specific against	Human (Homo sapiens)
Host	HEK293
Conjugate/Tag	HIS, Avi
Purity	> 95% as determined by Tris-Bis PAGE
Formula	Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Sequence	Thr32-His266
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	Dickkopf-1, Dkk1, SK
Available
Manufacturer - Conjugate / Tag	C-His-Avi
Shipping Temperature	Shipped at ambient temperature.
Storage Conditions	-20 to -80°C for 12 months as supplied from date of receipt.； -20 to -80°C for 3-6 months in unopened state after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please avoid freeze-thaw cycles.
Molecular Weight	The protein has a predicted MW of 28.5 kDa. Due to glycosylation, the protein migrates to 45-55 kDa based on Tris-Bis PAGE result.
Description	Recombinant Human DKK1 Protein is expressed from HEK293 with His tag and Avi tag at the C-terminal.It contains Thr32-His266.
Background	Dickkopf related protein 1 (Dkk-1) is the founding member of the Dickkopf family of proteins that includes Dkk-1, -2, -3, -4, and a related protein, Soggy. Dkk proteins are secreted proteins that contain two conserved cysteine-rich domains separated by a linker region. Dkk antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation.
Endotoxin	Less than 1EU per μg by the LAL method.
Formulation	Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Biotinylated	no
Activity	Immobilized Human DKK1, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human LRP-5, mFc Tag with the EC50 of 0.11μg/ml determined by ELISA. See testing image for detail.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Request Data Sheet

DKK-HM401-500UG-datasheet.pdf

DKK-HM401-500UG-safety-datasheet.pdf