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Recombinant Human ENPP7/NPP-7 Protein (His Tag)

Recombinant Human ENPP7/NPP-7 Protein (His Tag)

Item no.	ELS-PKSH031375-10ug
Manufacturer	Elabscience
Amount	10 ug
Quantity options	10 ug 20 ug 50 ug
Category	Metabolism Cholesterol & Lipid Metabolism Peptides & Proteins Recombinant Proteins Enzymes Fatty Acid Catabolism
Type	Proteins
Format	Lyophilized Powder
Specific against	Human (Homo sapiens)
Host	HEK293 cells
Conjugate/Tag	HIS
Purity	> 95 % as determined by reducing SDS-PAGE.
Sequence	Met 1-Ser 439
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	ALK-SMase, E-NPP7, NPP-7, NPP7
Similar products	ALK-SMase, NPP-7, E-NPP7, NPP7
Shipping Condition	Cool pack
Available
Manufacturer - Category	Recombinant Proteins / Others
Manufacturer - Conjugate / Tag	C-His
Shipping Temperature	This product is provided as lyophilized powder which is shipped with ice packs.
Storage Conditions	Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Calculated Molecular Weight	49.0 kDa
Observed Molecular Weight	55-60 kDa
Background	Ectonucleotide pyrophosphatase / phosphodiesterase family member 7, also known as Alkaline sphingomyelin phosphodiesterase, Intestinal alkaline sphingomyelinase, Alk-Smase, ENPP7 and NPP-7, is a single-pass type I membrane protein which belongs to thenucleotide pyrophosphatase / phosphodiesterase family. ENPP7 / NPP-7 is expressed in the intestines and human bile. ENPP7 / NPP-7 is localized at the surface of the microvillar membrane in small intestine enterocytes, as well as in endosome-like structures and in Golgi complex. The main function of ENPP7 / NPP-7 is to convert the dietary sphingomyelin into ceramide, the sphingolipid messengers via hydrolyzation. ENPP7 / NPP-7 is also reported to exert a phospholipase C activity toward palmitoyl lyso-phosphocholine. The activity of this enzyme is inhibited in a dose dependent manner by ATP, imidazole, orthovanadate and zinc ion. Further, It has been shown in studies that decreased levels of ENPP7 / NPP-7 may be associated with human colon cancer.
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method.
Reconstitution	Please refer to the printed manual for detailed information.
Formulation	Lyophilized from sterile PBS, pH 7.4 Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual.
Activity	Not validated for activity

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Request Data Sheet

ELS-PKSH031375-10ug-datasheet.pdf

ELS-PKSH031375-10ug-safety-datasheet.pdf