Recombinant Human NSE/ENO2 Protein (His Tag)

N-His

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

55 kDa

Gamma-enolase; also known as Enolase 2; belongs to the enolase family. The alpha/alpha homodimer of ENO2 is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle; and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons. During ontogenesis; there is a transition from the alpha/alpha homodimer to the alpha/beta heterodimer in striated muscle cells; and to the alpha/gamma heterodimer in nerve cells. Levels of ENO2 increase dramatically in cardiovascular accidents; cerebral trauma; brain tumors and Creutzfeldt-Jakob disease. ENO2 has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. It binds to cultured neocortical neurons and promotes cell survival in a calcium-dependent manner.

< 1.0 EU per μg of the protein as determined by the LAL method.

Lyophilized from a 0.2 μm filtered solution of 20mM PB, 8% Sucrose, 0.05% Tween 80, pH 7.0.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Recombinant Human NSE is produced by our E.coli expression system and the target gene encoding Met1-Leu434 is expressed with a 6His tag at the N-terminus.