Comparison

Recombinant Human EGF Protein

Item no. ELS-PKSH033687-50ug
Manufacturer Elabscience
Amount 50 ug
Quantity options 10 ug 50 ug 100 ug 500 ug
Category
Type Proteins
Format Lyophilized Powder
Applications Cell Culture
Specific against Human (Homo sapiens)
Host E.coli
Conjugate/Tag HIS
Purity > 95 % as determined by reducing SDS-PAGE.
Sequence Asn971-Arg1023
ECLASS 10.1 32160409
ECLASS 11.0 32160409
UNSPSC 12352202
Alias EGF, Epidermal Growth Factor, Pro-Epidermal Growth Factor, Urogastrone
Similar products EGF, Urogastrone, Epidermal Growth Factor, Pro-Epidermal Growth Factor
Shipping Condition Cool pack
Available
Manufacturer - Category
Recombinant Proteins / Cytokines
Manufacturer - Conjugate / Tag
C-His
Shipping Temperature
This product is provided as lyophilized powder which is shipped with ice packs.
Storage Conditions
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Calculated Molecular Weight
7.2 kDa
Observed Molecular Weight
9-11 kDa
Manufacturer - Research Areas
Signal Transduction, Cell biology, Cancer,
Background
Epidermal growth factor (EGF) is a small 53 amino acid residue long protein that contains three disulfide bridges. It is a small mitogenic protein that is thought to be involved in mechanisms such as normal cell growth, oncogenesis, and wound healing. EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. This protein shows both strong sequential and functional homology with human type-alpha transforming growth factor (hTGF alpha), which is a competitor for EGF receptor sites.
Abbreviation
EGF
Endotoxin
< 0.1 EU per μg of the protein as determined by the LAL method.
Reconstitution
Please refer to the printed manual for detailed information.
Formulation
Lyophilized from a 0.2 μm filtered solution of PBS, pH 8.0.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Activity
Measure by its ability to induce 3T3 cells proliferation. The ED50 for this effect is < 1.0 ng/mL. The specific activity of recombinant human EGF is approximately >1.0 x 106 IU/mg.
Protein Construction
Recombinant Human Epidermal Growth Factor is produced by our E.coli expression system and the target gene encoding Asn971-Arg1023 is expressed.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 50 ug
Available: In stock
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