Recombinant Human B7-H4/VTCN1 Protein (mFc Tag)

C-mFc

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

66-105 kDa

B7 Homolog 4 (B7-H4) is glycosylated member of the B7 family of immune costimulatory proteins. Mature human B7-H4 consists of a 235 amino acid (aa) extracellular domain (ECD) with two Ig-like V-type domains; a 21 aa transmembrane segment; and a 2 aa cytoplasmic tail. It is widely expressed; including in kidney; liver; lung; pancreas; placenta; prostate; spleen; testis and thymus. B7-H4 negatively regulates T-cell-mediated immune response by inhibiting T-cell activation; proliferation; cytokine production and development of cytotoxicity. When expressed on the cell surface of tumor macrophages; plays an important role; together with regulatory T-cells (Treg); in the suppression of tumor-associated antigen-specific T-cell immunity. It also involved in promoting epithelial cell transformation.

< 1.0 EU per μg of the protein as determined by the LAL method.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Recombinant Human B7-H4 is produced by our Mammalian expression system and the target gene encoding Phe29-Ala258 is expressed with a mFc tag at the C-terminus.