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Recombinant Mouse TNF-alpha/TNFA Protein

Recombinant Mouse TNF-alpha/TNFA Protein

Item no.	ELS-PKSM041176-50ug
Manufacturer	Elabscience
Amount	50 ug
Quantity options	10 ug 50 ug
Category	Oncology Neuroscience Neuroinflammation Metabolism Diabetes Molecular Targets for Neuroscience Aging & Neurological Disorders By Cell Type Peptides & Proteins Recombinant Proteins Cell Biology Obesity Cell Culture Cytokines Cell Status Necrosis Apoptosis
Type	Proteins
Format	Lyophilized Powder
Applications	Cell Culture
Specific against	Mouse (Murine, Mus musculus)
Host	E.coli
Conjugate/Tag	Untagged
Purity	> 95 % as determined by reducing SDS-PAGE.
Sequence	Asp89-Leu235
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	Cachectin, DIF, Membrane Form, TNF-Alpha, TNF-a, TNF-alpha, TNFSF2, TNFalpha, Tnf, Tnfa, Tnfsf1a, Tnfsf2, Tumor Necrosis Factor, Tumor Necrosis Factor Ligand Superfamily Member 2
Similar products	TNF-alpha, TNF-a, TNFalpha, TNFSF2, Cachectin, Tnf, DIF, TNF-Alpha, Tnfa, Tnfsf1a, Tumor Necrosis Factor, Tumor Necrosis Factor Ligand Superfamily Member 2, Membrane Form, Tnfsf2
Shipping Condition	Cool pack
Available
Manufacturer - Category	Recombinant Proteins / Cytokines
Manufacturer - Conjugate / Tag	None
Shipping Temperature	This product is provided as lyophilized powder which is shipped with ice packs.
Storage Conditions	Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Calculated Molecular Weight	16.4 kDa
Observed Molecular Weight	14 kDa
Background	Tumor Necrosis Factor (TNF) is a member of the Tumor Necrosis Factor family. TNF exists as a homotrimer and interacts with SPPL2B. TNF is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. TNF is a key cytokine in the development of several inflammatory disorders. It contributes to the development of type 2 diabetes throught its effects on insulin resistance and fatty acid metabolism.
Abbreviation	TNF-alpha; TNFA
Endotoxin	< 0.01 EU per μg of the protein as determined by the LAL method.
Reconstitution	Please refer to the printed manual for detailed information.
Formulation	Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual.
Activity	Measured in a cytotoxicity assay using L‑929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 2-8 pg/ml.
Protein Construction	Recombinant Mouse Tumor Necrosis Factor alpha is produced by our E.coli expression system and the target gene encoding Asp89-Leu235 is expressed.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Request Data Sheet

ELS-PKSM041176-50ug-datasheet.pdf

ELS-PKSM041176-50ug-safety-datasheet.pdf