Recombinant Streptomyces hygroscopicus Bar Protein

Recombinant Proteins / Others

This product is provided as lyophilized powder which is shipped with ice packs.

20.6 kDa

Phosphinothricin N-acetyltransferase (PAT) is an enzyme that acetylates the free NH2 group of L-phosphinothricin (L-PPT) in the presence of acetyl-CoA as a co-substrate. It is highly specific for L-PPT and does not acetylate other L-amino acids or structurally similar molecules. L-PPT is a glutamate analog that can inhibit glutamine synthetase activity in plants, resulting in the accumulation of ammonia to toxic levels and impairment of photosynthesis. The introduction of a PAT gene into a plant genome can confer resistance to glufosinate herbicide during post-emergent applications.

< 1.0 EU per μg of the protein as determined by the LAL method.

Lyophilized from a 0.2 μm filtered solution of 12.5mM Tris-HCl, 50mM NaCl, ‎‎5% Trehalose, 5% Mannitol, 0.01% Tween 80, 2mM DTT, 1mM EDTA, pH8.5.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Recombinant Streptomyces hygroscopicus Bar is produced by our E.coli expression system and the target gene encoding Met1-Ile183 is expressed.