Comparison

Recombinant Rat ASAM Protein (His Tag)

Item no. ELS-PKSR030312-100ug
Manufacturer Elabscience
Amount 100 ug
Quantity options 100 ug 50 ug
Category
Type Proteins
Format Lyophilized Powder
Applications Cell Culture
Specific against Rat (Rattus norvegicus)
Host HEK293 cells
Conjugate/Tag HIS
Purity > 85 % as determined by reducing SDS-PAGE.
Sequence Met1-Met232
ECLASS 10.1 32160409
ECLASS 11.0 32160409
UNSPSC 12352202
Alias ASAM, Acam, Clmp
Similar products ASAM, Clmp, Acam
Shipping Condition Cool pack
Available
Manufacturer - Category
Recombinant Proteins / Others
Manufacturer - Conjugate / Tag
C-His
Shipping Temperature
This product is provided as lyophilized powder which is shipped with ice packs.
Storage Conditions
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Calculated Molecular Weight
25.6 kDa
Observed Molecular Weight
37 kDa
Background
Adipocyte-specific adhesion molecule (ASAM), also known as ACAM and CLMP, is a type I transmembrane protein and a member of the CTX (cortical thymocyte marker in Xenopus) family within the immunoglobulin superfamily. ASAM protein is highly expressed in the small intestine and placenta, and is found at intermediate levels in the heart, skeletal muscle, colon, spleen, kidney, and lung, and appears in low levels in the liver and peripheral blood leukocytes as well. ASAM is a transmembrane component of tight junctions in epithelial cells that can mediate cell aggregation and regulate transepithelial resistance across polarized epithelial cells. In addition, its expression is strongly correlated with white adipose tissue (WAT) mass of human and rodents with obesity.
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method.
Reconstitution
Please refer to the printed manual for detailed information.
Formulation
Lyophilized from sterile PBS, pH 7.4
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Activity
Measured by the ability of the immobilized protein to support the adhesion of MS-1 cells. When 5 x 104 cells/well are added to Recombinant Rat ASAM coated plates (12.5 μg/mL with 100 μL/well), 35-70% cells will adhere after 1 hour incubation at 37°C.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 100 ug
Available: In stock
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