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Recombinant Rat TNF-alpha/TNFA Protein

Recombinant Rat TNF-alpha/TNFA Protein

Item no.	ELS-PKSR030443-5ug
Manufacturer	Elabscience
Amount	5 ug
Quantity options	20 ug 5 ug
Category	Oncology Neuroscience Neuroinflammation Metabolism Diabetes By Cell Type Peptides & Proteins Recombinant Proteins Cell Biology Obesity Cell Culture Cytokines Cell Status Necrosis Apoptosis
Type	Proteins
Format	Lyophilized Powder
Applications	Cell Culture
Specific against	Rat (Rattus norvegicus)
Host	E.coli
Conjugate/Tag	Untagged
Purity	> 93 % as determined by reducing SDS-PAGE.
Sequence	Leu 80-Leu 235
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	MGC124630, RATTNF, TNF-alpha, Tnf, Tnfa
Similar products	TNF-alpha, Tnf, Tnfa, MGC124630, RATTNF
Shipping Condition	Cool pack
Available
Manufacturer - Category	Recombinant Proteins / Cytokines
Manufacturer - Conjugate / Tag	None
Shipping Temperature	This product is provided as lyophilized powder which is shipped with ice packs.
Storage Conditions	Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Calculated Molecular Weight	17.4 kDa
Observed Molecular Weight	15 kDa
Background	Tumor necrosis factor alpha (TNF-alpha), also known as TNF, TNFA or TNFSF2, is the prototypic cytokine of the TNF superfamily, and is a multifunctional molecule involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. Two receptors, TNF-R1 (TNF receptor type 1; CD120a; p55/60) and TNF-R2 (TNF receptor type 2; CD120b; p75/80), bind to TNF-alpha. TNF-alpha protein is produced mainly by macrophages, and large amounts of this cytokine are released in response to lipopolysaccharide, other bacterial products, and Interleukin-1 (IL-1). TNF-alpha is involved in fighting against the tumorigenesis, thus, is regarded as a molecular insight in cancer treatment.
Endotoxin	Please contact us for more information.
Reconstitution	Please refer to the printed manual for detailed information.
Formulation	Lyophilized from sterile PBS, pH 7.4 Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual.
Activity	Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of metabolic inhibitor actinomycin D. The ED50 for this effect is typically 20-80 pg/ml.
Protein Construction	A DNA sequence encoding the mature form of rat TNFalpha (P16599) (Leu 80-Leu 235) was expressed and purified, with an initial Met at the N-terminus.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Request Data Sheet

ELS-PKSR030443-5ug-datasheet.pdf

ELS-PKSR030443-5ug-safety-datasheet.pdf