Product Description |
TOP1 Protein, pivotal in cellular processes, alleviates DNA supercoiling and torsional tension during replication and transcription. It achieves this by transiently cleaving and rejoining one DNA strand, introducing a break via transesterification. This forms a DNA-(3'-phosphotyrosyl)-enzyme intermediate, expelling a 5'-OH DNA strand and allowing rotation. In the final step, religation restores the DNA phosphodiester backbone. TOP1, beyond DNA topology, regulates alternative splicing, impacting tissue factor (F3) pre-mRNA. Additionally, it contributes to circadian transcription, modifying chromatin structure around BMAL1 promoter ROREs. TOP1 Protein, Human (575a.a, sf9, His) is the recombinant human-derived TOP1 protein, expressed by Sf9 insect cells, with N-His labeled tag. The total length of TOP1 Protein, Human (575a.a, sf9, His) is 575 a.a., with molecular weight of ~70.4 kDa. |