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Human IgG4 Fc Protein, Tag Free (MALS & SPR verified)

Human IgG4 Fc Protein, Tag Free (MALS & SPR verified)

Item no.	IG4-H5205-200ug
Manufacturer	ACROBiosystems
Amount	200 ug
Quantity options	1 mg 200 ug
Category	Peptides & Proteins Recombinant Proteins
Type	Proteins Recombinant
Format	Solid
Specific against	Human (Homo sapiens)
Host	HEK293
Conjugate/Tag	Unconjugated
Purity	95%
Citations	(1) Frangione B., et al., 1969, Nature 221:145-148. (2) Kristiansen T.Z., et al., 2004, Mol. Cell. Proteomics 3:715-728. (3) Chen R., et al., 2009, J. Proteome Res. 8:651-661.
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	IGHG4
Shipping Condition	Room temperature
Available
Manufacturer - Category	Protein / Other Recombinant Proteins
Manufacturer - Conjugate / Tag	Native, Unconjugated
Shipping Temperature	RT
Storage Conditions	-20°C
Molecular Weight	25.8 kDa
Manufacturer - Format	Powder
Description	Human IgG4 Fc, Tag Free (IG4-H5205) is expressed from human 293 cells (HEK293). It contains AA Glu 99 - Lys 327 (Accession # P01861).
Background	Immunoglobulin G4 (IgG4) is a member of many immunoglobulin G developed and secreted by effective B cells. In wake of cutting by pepsin, IgG is divided into two F(ab)s with one antigen binding site and a high conserved Fc segment. The Fc segment bears a highly conserved N-glycosylation site. Ig gamma-4 chain Fc region contains two constant regions of IgG4 H chain (CH2, CH3).
Molecule	IgG4 Fc
Exp Region	Glu 99 - Lys 327
Characteristics	This protein carries no "tag". The protein has a calculated MW of 25.8 kDa. The protein migrates as 30-33 kDa under reducing (R) condition, and 29 kDa and 55-60 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.
Endotoxin	1.0 EU per μg
Buffer	Tris with Glycine, Arginine and NaCl, pH7.5
Stability	● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution._x000D_For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Protectant	trehalose

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Request Data Sheet

IG4-H5205-200ug-datasheet.pdf

IG4-H5205-200ug-safety-datasheet.pdf