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Biotinylated Mouse IgG2a Fc Protein, Avitag™ (MALS verified)

Biotinylated Mouse IgG2a Fc Protein, Avitag™ (MALS verified)

Item no.	IGA-M8210-25ug
Manufacturer	ACROBiosystems
Amount	25 ug
Quantity options	25 ug 500 ug
Category	Peptides & Proteins Recombinant Proteins
Type	Proteins Recombinant
Format	Solid
Specific against	Mouse (Murine, Mus musculus)
Host	HEK293
Conjugate/Tag	Biotin, Avi
Purity	95%
Citations	(1) Sikorav J.-L., et al., 1980, Nucleic Acids Res. 8:3143-3155. (2) Ollo R., et al., 1981, Proc. Natl. Acad. Sci. U.S.A. 78:2442-2446.
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	IgG2A
Shipping Condition	Room temperature
Available
Manufacturer - Category	Protein / MABSol® Biotin Labeled Proteins
Manufacturer - Conjugate / Tag	C-Avi, Biotin-labeled
Shipping Temperature	RT
Storage Conditions	-20°C
Molecular Weight	28.2 kDa
Manufacturer - Format	Powder
Description	Biotinylated Mouse IgG2a Fc, Avitag (IGA-M8210) is expressed from human 293 cells (HEK293). It contains AA Glu 98 - Lys 330 (Accession # P01863).
Background	Immunoglobulin G2 (IgG2) is a member of many immunoglobulin G developed and secreted by effective B cells. In wake of cutting by pepsin, IgG is divided into two F(ab)s with one antigen binding site and a high conserved Fc segment. The Fc segment bears a highly conserved N-glycosylation site. There are two members of IgG2: IgG2a and IgG2b. It was found that IgG2a was superior to IgG1 in activating complement. The glycosylation of the circulating immunoglobulin-γ (IgG) antibody molecules changes in rheumatoid arthritis.
Molecule	IgG2a Fc
Exp Region	Glu 98 - Lys 330
Characteristics	This protein carries an Avi tag (Avitag™) at the C-terminus. The protein has a calculated MW of 28.2 kDa. The protein migrates as 32-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin	1.0 EU per μg
Buffer	Tris with Glycine, Arginine and NaCl, pH7.5
Stability	● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution._x000D_For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Protectant	trehalose

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Request Data Sheet

IGA-M8210-25ug-datasheet.pdf

IGA-M8210-25ug-safety-datasheet.pdf