Comparison

Human Integrin alpha V beta 5 (ITGAV&ITGB5) Heterodimer Protein, His Tag&Tag Free European Partner

Item no. IT5-H52W5-100ug
Manufacturer ACROBiosystems
Amount 100 ug
Quantity options 100 ug 500 ug x 2
Category
Type Proteins Recombinant
Format Solid
Specific against Human (Homo sapiens)
Host HEK293
Conjugate/Tag Unconjugated, HIS
Purity 95%
Citations (1) Suzuki, S. et al., 1990,Proc. Natl. Acad. Sci. USA 87:5354-8. (2) Memmo, L.M. and P. McKeown-Longo, 1998, J. Cell Sci.111:425-33. (3) Desgrosellier, J.S. and D.A. Cheresh, 2010, Nat. Rev. Cancer 10:9-22. (4) Luzina, I.G. et al., 2009, Arthritis Rheum. 60:1530-9.
NCBI NP_002201.1 (ITGAV) , NP_002204.2 (ITGB5)
ECLASS 10.1 32160409
ECLASS 11.0 32160409
UNSPSC 12352202
Alias Integrin alpha V beta 5,ITGAV&ITGB5
Shipping Condition Room temperature
Available
Manufacturer - Category
Protein / Bio-Markers & CD Antigens
Manufacturer - Conjugate / Tag
C-10xHis | Native, Unconjugated
Shipping Temperature
RT
Storage Conditions
-20°C
Molecular Weight
112.9 kDa (ITGAV) & 81.9 kDa (ITGB5)
Manufacturer - Format
Powder
Description
Human ITGAV&ITGB5 Heterodimer Protein, His Tag&Tag Free (IT5-H52W5) is expressed from human 293 cells (HEK293). It contains AA Phe 31 - Val 992 (ITGAV) & Gly 24 - Asn 719 (ITGB5) (Accession # NP_002201.1 (ITGAV) & NP_002204.2 (ITGB5)).
Background
Integrin alpha V beta 5 (ITGAV & ITGB5) is expressed on a wide variety of cell types including keratinocytes, fibroblasts, adhesive monocytes, embryonic stem cells, and select endothelium and epithelium. ITGAV & ITGB5 binds ligands containing an RGD motif, notably vitronectin. Growth factors that increase PKC activity, such as VEGF or TGF alpha, promote ITGAV & ITGB5-mediated angiogenesis while alpha V beta 3, which may be expressed in the same cell, responds to FGF-basic and TNF alpha. An inhibitor of both down regulates tumor angiogenesis. During lung inflammation, up regulation of ITGAV & ITGB5 on myofibroblasts or infiltrating lymphocytes may contribute to fibrosis by freeing TGF beta from latency.
Molecule
Integrin alpha V beta 5
Exp Region
Phe 31 - Val 992 (ITGAV) & Gly 24 - Asn 719 (ITGB5)
Characteristics
Human ITGAV&ITGB5 Heterodimer Protein, His Tag&Tag Free, produced by co-expression of ITGAV and ITGB5, has a calculated MW of 112.9 kDa (ITGAV) and 81.9 kDa (ITGB5). Subunit ITGAV is fused with polyhistidine tag at the C-terminus and followed by a acidic tail and subunit ITGB5 contains no tag but a basic tail at the C-terminus. The non-reducing (NR) protein migrates as 130-150 kDa (ITGAV) and 85-95 kDa (ITGB5) respectively due to glycosylation.
Endotoxin
1.0 EU per μg
Buffer
50 mM Tris, 150 mM NaCl, pH7.5
Stability
● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution._x000D_For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Protectant
trehalose

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 100 ug
Available: In stock
available

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