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Biotinylated Human Integrin alpha V beta 5 (ITGAV&ITGB5) Heterodimer Protein, His,Avitag™&Tag Free (MALS verified)

Biotinylated Human Integrin alpha V beta 5 (ITGAV&ITGB5) Heterodimer Protein, His,Avitag™&Tag Free (MALS verified)

Item no.	IT5-H82Wa-200ug
Manufacturer	ACROBiosystems
Amount	200 ug
Quantity options	200 ug 25 ug
Category	Peptides & Proteins Recombinant Proteins
Type	Proteins Recombinant
Format	Solid
Specific against	Human (Homo sapiens)
Host	HEK293
Conjugate/Tag	Biotin, HIS, Avi
Purity	90%
Citations	(1) Suzuki, S. et al., 1990,Proc. Natl. Acad. Sci. USA 87:5354-8. (2) Memmo, L.M. and P. McKeown-Longo, 1998, J. Cell Sci.111:425-33. (3) Desgrosellier, J.S. and D.A. Cheresh, 2010, Nat. Rev. Cancer 10:9-22. (4) Luzina, I.G. et al., 2009, Arthritis Rheum. 60:1530-9.
NCBI	NP_002201.1 (ITGAV) , NP_002204.2 (ITGB5)
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	Integrin alpha V beta 5,ITGAV&ITGB5
Shipping condition	Room temperature
Available
Manufacturer - Category	Protein / MABSol® Biotin Labeled Proteins
Manufacturer - Conjugate / Tag	C-10×His & C-Avi \| Native, Biotin-labeled
Shipping Temperature	RT
Storage Conditions	-20°C
Molecular Weight	114.7 kDa (ITGAV) & 81.9 kDa (ITGB5)
Description	Biotinylated Human ITGAV&ITGB5 Heterodimer Protein, His, Avitag&Tag Free (IT5-H82Wa) is expressed from human 293 cells (HEK293). It contains AA Phe 31 - Val 992 (ITGAV) & Gly 24 - Asn 719 (ITGB5) (Accession # P06756-1 (ITGAV) & P18084-1 (ITGB5)).
Background	Integrin alpha V beta 5 (ITGAV & ITGB5) is expressed on a wide variety of cell types including keratinocytes, fibroblasts, adhesive monocytes, embryonic stem cells, and select endothelium and epithelium. ITGAV & ITGB5 binds ligands containing an RGD motif, notably vitronectin. Growth factors that increase PKC activity, such as VEGF or TGF alpha, promote ITGAV & ITGB5-mediated angiogenesis while alpha V beta 3, which may be expressed in the same cell, responds to FGF-basic and TNF alpha. An inhibitor of both down regulates tumor angiogenesis. During lung inflammation, up regulation of ITGAV & ITGB5 on myofibroblasts or infiltrating lymphocytes may contribute to fibrosis by freeing TGF beta from latency.
Molecule	Integrin alpha V beta 5
Exp Region	Phe 31 - Val 992 (ITGAV) & Gly 24 - Asn 719 (ITGB5)
Characteristics	Biotinylated Human ITGAV&ITGB5 Heterodimer Protein, His, Avitag&Tag Free, produced by co-expression of ITGAV and ITGB5, has a calculated MW of 114.7 kDa (ITGAV) and 81.9 kDa (ITGB5). Subunit ITGAV is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and an Avi tag (Avitag™) and subunit ITGB5 contains no tag but a basic tail at the C-terminus. The non-reducing (NR) protein migrates as 130-170 kDa (ITGAV) and 85-100 kDa (ITGB5) when calibrated against Star Ribbon Pre-stained Protein Marker respectively due to glycosylation.
Endotoxin	1.0 EU per μg
Buffer	50 mM Tris, 150 mM NaCl, pH7.5
Stability	-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution._x000D_For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Protectant	trehalose

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Request Data Sheet

IT5-H82Wa-200ug-datasheet.pdf

IT5-H82Wa-200ug-safety-datasheet.pdf