Rat Integrin alpha V beta 5 (ITGAV&ITGB5) Heterodimer Protein, His Tag&Tag Free

Protein

RT

112.3 kDa | 81.7 kDa

Integrin alpha V beta 5 (ITGAV & ITGB5) is expressed on a wide variety of cell types including keratinocytes, fibroblasts, adhesive monocytes, embryonic stem cells, and select endothelium and epithelium. ITGAV & ITGB5 binds ligands containing an RGD motif, notably vitronectin. Growth factors that increase PKC activity, such as VEGF or TGF alpha, promote ITGAV & ITGB5-mediated angiogenesis while alpha V beta 3, which may be expressed in the same cell, responds to FGF-basic and TNF alpha. An inhibitor of both down regulates tumor angiogenesis. During lung inflammation, up regulation of ITGAV & ITGB5 on myofibroblasts or infiltrating lymphocytes may contribute to fibrosis by freeing TGF beta from latency.

Integrin alpha V beta 5

Rat ITGAV&ITGB5 Heterodimer, His Tag&Tag Free, produced by co-expression of ITGAV and ITGB5, has a calculated MW of 112.3 kDa (ITGAV) & 81.6 kDa (ITGB3). Subunit ITGAV is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and subunit ITGB3 contains no tag but a basic tail at the C-terminus. The protein migrates as 125-145 kDa (ITGAV) and 85-95 kDa (ITGB3) when calibrated against Star Ribbon Pre-stained Protein Marker under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.

50 mM Tris, 150 mM NaCl, pH7.5

trehalose