Comparison

Biotinylated Human Integrin alpha V beta 8 (ITGAV&ITGB8) Heterodimer Protein, His,Avitag™&Tag Free (MALS verified) European Partner

Item no. IT8-H82W5-25ug
Manufacturer ACROBiosystems
Amount 25 ug
Quantity options 200 ug 25 ug
Category
Type Proteins Recombinant
Format Solid
Specific against Human (Homo sapiens)
Host HEK293
Conjugate/Tag Biotin, HIS, Avi
Purity 95%
Citations (1) Moyle, M. et al., 1991, J. Biol. Chem. 266:19650-8. (2) Nishimura SL., et al., 1994, J Biol Chem. 269(46):28708-15. (3) Araya, J. et al., 2006, Am. J. Pathol. 169:405-15. (4) Mu,D.et al.,2002, J. Cell Biol.157:493-507.
NCBI NP_002201.1 (ITGAV) , NP_002205.1 (ITGB8)
ECLASS 10.1 32160409
ECLASS 11.0 32160409
UNSPSC 12352202
Alias Integrin alpha V beta 8,ITGAV&ITGB8
Shipping Condition Room temperature
Available
Manufacturer - Category
Protein / MABSol® Biotin Labeled Proteins
Manufacturer - Conjugate / Tag
C-10xHis, C-Avi | Native, Biotin-labeled
Shipping Temperature
RT
Storage Conditions
-20°C
Molecular Weight
114.7 kDa (ITGAV) & 76.5 kDa (ITGB8)
Manufacturer - Format
Powder
Description
Biotinylated Human ITGAV&ITGB8 Heterodimer Protein, His, Avitag&Tag Free (IT8-H82W5) is expressed from human 293 cells (HEK293). It contains AA Phe 31 - Val 992 (ITGAV) & Glu 43 - Arg 684 (ITGB8) (Accession # NP_002201.1 (ITGAV) & NP_002205.1 (ITGB8)).
Background
Integrin alpha V beta 8 (ITGAV & ITGB8 or ITGAVB8) is expressed in yolk sac, placenta, brain perivascular astrocytes, Schwann cells, renal glomerular mesangial cells and pulmonary epithelial cells. Unlike other alpha V integrins, ITGAVB8 does not appear to assume different activation states, and the cytoplasmic tail does not connect to the cytoskeleton. It binds ligands containing an RGD motif, including vitronectin, fibrin and the latency associated peptide (LAP) of the latent TGF-beta complex. High affinity binding of alpha V beta 8 to LAP allows proteolytic cleavage by MT1-MMP, which releases active TGF-beta. This mechanism differs from that of alpha V beta 6, the other alpha V integrin which can activate TGF-beta from latency through non-proteolytic mechanisms. Downstream effects of TGF-beta activation include control of cell growth and associated vascularization.
Molecule
Integrin alpha V beta 8
Exp Region
Phe 31 - Val 992 (ITGAV) & Glu 43 - Arg 684 (ITGB8)
Characteristics
Biotinylated Human ITGAV&ITGB8 Heterodimer Protein, His, Avitag&Tag Free, produced by co-expression of ITGAV and ITGB8, has a calculated MW of 114.7 kDa (ITGAV) and 76.5 kDa (ITGB8). Subunit ITGAV is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and an Avi tag (Avitag™) and subunit ITGB8 contains no tag but a basic tail at the C-terminus. The non-reducing (NR) protein migrates as 135 kDa (ITGAV) and 80 kDa (ITGB8) respectively due to glycosylation.
Endotoxin
1.0 EU per μg
Buffer
50 mM Tris, 150 mM NaCl, pH8.0
Stability
● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution._x000D_For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Protectant
trehalose

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 25 ug
Available: In stock
available

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