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Chimeric HLA-A*02:01 (mα3) &B2M&WT-1 (RMFPNAPYL) Tetramer Protein (MHC-HM414T)

Chimeric HLA-A*02:01 (mα3) &B2M&WT-1 (RMFPNAPYL) Tetramer Protein (MHC-HM414T)

Item no.	MHC-HM414T-100UG
Manufacturer	KactusBio
Amount	100 ug
Quantity options	100 ug 500 ug
Category	Oncology By Cell Type Carcinoma Glioma Hematology Leukemia Lymphoma Myeloma Melanoma Peptides & Proteins Recombinant Proteins Adenocarcinoma Myelofibrosis Mesothelioma Astrocytoma By Organ Heart & Blood Cancer Blastom
Type	Proteins Recombinant
Format	Lyophilized
Specific against	Human (Homo sapiens), Mouse (Murine, Mus musculus)
Host	HEK293
Conjugate/Tag	HIS, Avi
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Formula	Supplied as 0.22μm filtered solution in PBS (pH 7.4).
Sequence	Gly25-Thr206(Human HLA-A*02:01 α1&α2) & Asp207-Glu299(Mouse H-2Ld α3), Ile21-Met119(B2M)and RMFPNAPYL peptide
NCBI	RMFPNAPYL
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	MHC,WT1,RMF,AWT1,GUD,NPHS4GUD,WAGR,WIT-2,WT33
Shipping Condition	Room temperature
Available
Manufacturer - Type	Recombinant Protein
Manufacturer - Targets	Chimeric HLA-A*02:01 (mα3) &B2M&WT-1 (RMFPNAPYL)
Manufacturer - Conjugate / Tag	C-His-Avi
Shipping Temperature	Shipped at ambient temperature.
Storage Conditions	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Molecular Weight	The protein has a predicted MW of 258 kDa. Due to glycosylation, the protein migrates to 260-265 kDa under Non reducing (N) condition based on Bis-Tris PAGE result.
Description	Recombinant Chimeric HLA-A02:01(mα3)&B2M&WT-1 (RMFPNAPYL) Tetramer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus, tetramer is assembled by biotinylated monomer and streptavidin.It contains Gly25-Thr206(Human HLA-A02:01 α1&α2)&Asp207-Glu299(Mouse H-2Ld α3), Ile21-Met119 (B2M) and RMFPNAPYL peptide.
Background	The WT1 protein plays a role in cell growth, the process by which cells mature to perform specific functions (differentiation), and the self-destruction of cells (apoptosis). WT1 is differentially expressed in serous, endometrioid, clear cell, and mucinous carcinomas of the peritoneum, fallopian tube, ovary, and endometrium.The Human HLA-A0201 WT-1 (RMFPNAPYL) complex Protein is a complex of HLA-A0201 of the MHC Class I, B2M and RMFPNAPYL peptide of the WT-1.
Endotoxin	Less than 1 EU per ug by the LAL method.
Formulation	Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1mg/ml for 500μg or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Biotinylated	no

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Request Data Sheet

MHC-HM414T-100UG-datasheet.pdf

MHC-HM414T-100UG-safety-datasheet.pdf