Comparison

Human HLA-A*02:01&B2M&PRAME (ALYVDSLFFL) Tetramer Protein (MHC-HM447T)

Item no. MHC-HM447T-500UG
Manufacturer KactusBio
Amount 500 ug
Quantity options 100 ug 500 ug
Category
Type Proteins Recombinant
Format Lyophilized
Specific against Human (Homo sapiens)
Host HEK293
Conjugate/Tag HIS, Avi
Purity > 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC
Sequence Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and ALYVDSLFFL peptide
NCBI ALYVDSLFFL
ECLASS 10.1 32160409
ECLASS 11.0 32160409
UNSPSC 12352202
Available
Manufacturer - Product Type
Recombinant Protein
Description
Recombinant Human HLA-A*02:01&B2M&PRAME (ALYVDSLFFL) Tetramer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus, tetramer is assembled by biotinylated monomer and streptavidin.It contains Gly25-Thr305 (HLA-A*02:01), Ile21-Met119 (B2M) and ALYVDSLFFL peptide.
Background
PRAME (PReferentially expressed Antigen in MElanoma) is a melanoma-associated antigen expressed in cutaneous and ocular melanomas and some other malignant neoplasms, while its expression in normal tissue and benign tumors is limited.
Endotoxin
Less than 1 EU per μg by the LAL method.
Molecular Weight
The protein has a predicted MW of 258 kDa. Due to glycosylation, the protein migrates to 260-265 kDa under Non reducing (N) condition based on Bis-Tris PAGE result.
Target
HLA-A*02:01&B2M&PRAME (ALYVDSLFFL) Tetramer
Manufacturer - Tag
C-His-Avi
Stability and Storage
-20 to -80°C for 12 months as supplied from date of receipt; -80°C for 3-6 months after reconstitution; 2-8°C for 2-7 days after reconstitution; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, 100mM L-arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Biotinylated
no

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 500 ug
Available: In stock
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