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Biotinylated Human HLA-A*02:01&B2M&CMV pp65 (NLVPMVATV) Monomer Protein (MHC-HM458B)

Biotinylated Human HLA-A*02:01&B2M&CMV pp65 (NLVPMVATV) Monomer Protein (MHC-HM458B)

Item no.	MHC-HM458B-100UG
Manufacturer	KactusBio
Amount	100 ug
Quantity options	100 ug 500 ug
Category	Oncology By Cell Type Carcinoma Glioma Hematology Leukemia Lymphoma Myeloma Melanoma Peptides & Proteins Recombinant Proteins Adenocarcinoma Myelofibrosis Mesothelioma Astrocytoma By Organ Heart & Blood Cancer Blastom
Type	Proteins Recombinant
Format	Lyophilized
Specific against	Human (Homo sapiens)
Host	HEK293
Conjugate/Tag	HIS, Avi
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Sequence	Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and NLVPMVATV peptide
NCBI	NLVPMVATV
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	MHC,HLA-A*02:01,CMV pp65
Shipping Condition	Room temperature
Available
Manufacturer - Type	Recombinant Protein
Manufacturer - Targets	HLA-A*02:01&B2M&CMV pp65 (NLVPMVATV)
Manufacturer - Conjugate / Tag	C-His-Avi
Shipping Temperature	Shipped at ambient temperature.
Storage Conditions	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Molecular Weight	The protein has a predicted MW of 50.35 kDa. Due to glycosylation, the protein migrates to 53-63 kDa based on Bis-Tris PAGE result.
Description	Recombinant Biotinylated Human HLA-A02:01&B2M&CMV pp65 (NLVPMVATV) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly25-Thr305(HLA-A02:01), Ile21-Met119(B2M) and NLVPMVATV peptide.
Background	Human cytomegalovirus (CMV) is one of the major causes of severe complications in immunocompromised patients, such as recipients of hematopoietic cells, solid organ transplantation, and HIV-infected individuals. CMV is also a major pathogen in congenital infection. It can cause life-threatening diseases and severe neurological sequelae in newborn infants. CMV phosphoprotein pp65 is thought to be a major antigen for CMV-specific cellular immunity.
Endotoxin	Less than 1 EU per μg by the LAL method.
Formulation	Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1mg/ml for 500μg or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Biotinylated	yes

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Request Data Sheet

MHC-HM458B-100UG-datasheet.pdf

MHC-HM458B-100UG-safety-datasheet.pdf