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Biotinylated Human Nectin-4 Protein, His,Avitag™ (MALS verified) European Partner

Item no. NE4-H82E7-25ug
Manufacturer ACROBiosystems
Amount 25 ug
Quantity options 200 ug 25 ug
Category
Type Proteins Recombinant
Format Solid
Specific against Human (Homo sapiens)
Host HEK293
Conjugate/Tag Biotin, HIS, Avi
Purity 95%
Citations (1) Reymond N., et al. 2001, J Biol Chem., 276(46), 43205-15. (2) Ma J., et al. 2016, Onco Targets Ther., 9, 183-90. (3) Nabih ES., et al. 2014, Biomarkers., 19(6), 498-504.
NCBI NP_112178.2
ECLASS 10.1 32160409
ECLASS 11.0 32160409
UNSPSC 12352202
Alias NECTIN4,LNIR, PRR4, PVRL4
Shipping Condition Room temperature
Available
Manufacturer - Category
Protein / MABSol® Biotin Labeled Proteins
Manufacturer - Conjugate / Tag
C-10xHis & C-Avi, Biotin-labeled
Shipping Temperature
RT
Storage Conditions
-20°C
Molecular Weight
37.8 kDa
Manufacturer - Format
Powder
Description
Biotinylated Human Nectin-4, His, Avitag (NE4-H82E7) is expressed from human 293 cells (HEK293). It contains AA Gly 32 - Ser 349 (Accession # Q96NY8-1).
Background
Nectin-4 (gene name PVRL4, poliovirus receptor-like 4) is a 66 kDa type I transmembrane glycoprotein belonging to the Nectin family of Ig superfamily proteins. Nectins are cell adhesion molecules that play a key role in various biological processes such as polarity, proliferation, differentiation and migration, for epithelial, endothelial, immune and neuronal cells, during development and adult life. Nectin-4 is a tumor-associated antigen in 50%, 49% and 86%o of breast, ovarian and lung carcinomas, respectively, mostly on tumors of bad prognosis. Its expression is not detected in the corresponding normal tissues.
Molecule
Nectin-4
Exp Region
Gly 32 - Ser 349
Characteristics
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™). The protein has a calculated MW of 37.8 kDa. The protein migrates as 40-45 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
1.0 EU per μg
Buffer
PBS, pH 7.4
Stability
● -20°C to -70°C for 12 months in lyophilized state;
● -70°C for 3 months under sterile conditions after reconstitution._x000D_For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Protectant
trehalose

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 25 ug
Available: In stock
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