Comparison

Recombinant human PCMT1 protein

Item no. NKM-ATGP0578-20ug
Manufacturer NKMAX
Amount 20 ug
Quantity options 100 ug 20 ug 500 ug
Category
Type Proteins Recombinant
Format Liquid
Applications SDS-PAGE
Specific against Human (Homo sapiens)
Host E.coli
Conjugate/Tag HIS
Purity > 95% by SDS-PAGE
Sequence <MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGS>MAWK SGGASHSELI HNLRKNGIIK TDKVFEVMLA TDRSHYAKCN PYMDSPQSIG FQATISAPHM HAYALELLFD QLHEGAKALD VGSGSGILTA CFARMVGCTG KVIGIDHIKE LVDDSINNVR KDDPTLLSSG RVQLVVGDGR MGYAEEAPYD AIHVGAAAPV VPQALIDQLK PGGRLILPVG PAGGNQMLEQ
Citations Kagan RM., et al. (1997) Comp Biochem Physiol B Biochem Mol Biol. 117(3):379-85.
Maeda H, et al. Rapid survey of four Asp isomers in disease-related proteins by LC-MS combined with commercial enzymes. (Anal Chem. 2015){25479244}
Lyon YA, et al. Differences in α-Crystallin isomerization reveal the activity of protein isoaspartyl methyltransferase (PIMT) in the nucleus and cortex of human lenses. (Exp Eye Res. 2018){https://pubmed.ncbi.nlm.nih.gov/29571628/}
Takata T, et al. Site-specific rapid deamidation and isomerization in human lens αA-crystallin in vitro. (Protein Sci. 2020){https://pubmed.ncbi.nlm.nih.gov/31930615/}
Magami K, et al. Isomerization of Asp is essential for assembly of amyloid-like fibrils of αA-crystallin-derived peptide. (PLoS One. 2021){https://pubmed.ncbi.nlm.nih.gov/33857260/}
NCBI AAH08748.1
ECLASS 10.1 32160409
ECLASS 11.0 32160409
UNSPSC 12352202
Alias Protein-L-isoaspartate D-aspartate O-methyltransferase,PIMT,L-isoaspartyl protein carboxyl methyltransferase,Protein L-isoaspartyl/D-aspartyl methyltransferase,Protein-beta-aspartate methyltransferase
Similar products PCMT, PIMT, L isoaspartyl/D aspartyl methyltransferase, PCMT 1, Protein beta aspartate methyltransferase, Protein L isoaspartate (D aspartate) O methyltransferase, Protein L isoaspartyl/D aspartyl methyltransferase, Protein-L-isoaspartate O-methyltransferase, Protein-L-isoaspartate O-methyltransferase L isoaspartyl protein carboxyl methyltransferase, ATGP0578-10ug, ATGP0578-20ug, ATGP0578-50ug, ATGP0578-100ug, ATGP0578-250ug, ATGP0578-500ug, ATGP0578-1mg, ATGP0578-10, ATGP0578-20, ATGP0578-50, ATGP0578-100, ATGP0578-250, ATGP0578-500, ATGP0578-1
Available
Manufacturer - Type
Recombinant Protein
Storage Conditions
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Molecular Weight
28.7 kDa (263aa) confirmed by MALDI-TOF
Description
PCMT1, also known as L-isoaspartyl protein carboxyl methyltransferase, is an enzyme that catalyses the methyl esterification of L-isoaspartyl and D-aspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. This protein plays a role in the repair and/or degradation of damaged proteins. Recombinant human PCMT1 protien, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Concentration
1mg/ml (determined by Bradford assay)
Formulation
Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol 0.1M NaCl
Antigen Species
Human
Gene Category
Enzymes & Proteases
Domain
1-227aa
Species of Protein
Human
Manufacturer - Search Terms
PCMT, Protein-L-isoaspartate O-methyltransferase, Protein-L-isoaspartate O-methyltransferase L isoaspartyl protein carboxyl methyltransferase, L isoaspartyl/D aspartyl methyltransferase, PCMT 1, PIMT, Protein beta aspartate methyltransferase, Protein L isoaspartate (D aspartate) O methyltransferase, Protein L isoaspartyl/D aspartyl methyltransferase, ATGP0578-10ug, ATGP0578-20ug, ATGP0578-50ug, ATGP0578-100ug, ATGP0578-250ug, ATGP0578-500ug, ATGP0578-1mg, ATGP0578-10, ATGP0578-20, ATGP0578-50, ATGP0578-100, ATGP0578-250, ATGP0578-500, ATGP0578-1

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 20 ug
Available: In stock
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