Recombinant human RDBP/NELFE protein

Proteins

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

RDBP, also known as NELF-E, is a putative RNA binding protein. This protein is one of the five components of the multisubunit NELF complex that cooperates with DSIF to repress RNA polymerase II elongation. Control of transcription elongation requires a complex interplay between positive transcription elongation factor b and negative transcription elongation factors, DSIF and NELF. DSIF and NELF, act as negative transcription elongation factors by increasing the time the polymerase spends at pause sites. RDBP has a functional RNA-binding domain, whose mutations impair transcription repression without affecting known protein-protein interactions. Recombinant human RDBP protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 50% glycerol, 1mM DTT, 0.1mM PMSF

Epigenomics (Transcription & Translation)

Human