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Recombinant E.coli D-lactate dehydrogenase/LDHA protein

Recombinant E.coli D-lactate dehydrogenase/LDHA protein

Item no.	NKM-ATGP3236-100ug
Manufacturer	NKMAX
Amount	100 ug
Quantity options	100 ug 10 ug 20 ug 250 ug 500 ug 50 ug
Category	Peptides & Proteins Recombinant Proteins Cell Biology Cell Status Toxicity & Cytotoxicity
Type	Proteins Recombinant
Format	Liquid
Applications	SDS-PAGE
Specific against	Escherichia coli (E.coli)
Host	E.coli
Conjugate/Tag	HIS
Purity	> 95% by SDS-PAGE
Sequence	<MGSSHHHHHH SSGLVPRGSH MGSH>MKLAVY STKQYDKKYL QQVNESFGFE LEFFDFLLTE KTAKTANGCE AVCIFVNDDG SRPVLEELKK HGVKYIALRC AGFNNVDLDA AKELGLKVVR VPAYDPEAVA EHAIGMMMTL NRRIHRAYQR TRDANFSLEG LTGFTMYGKT AGVIGTGKIG VAMLRILKGF GMRLLAFDPY PSAAALELGV EYVDLPTLFS ESDVISLHCP
Citations	Atlante, A., et al. (2005) Biochim. Biophys. Acta 1708 (1): 13-22. Martin Engqvist, et al. (2009) J Biol Chem. 284 (September 11): 25026-25037.
NCBI	NP_415898.1
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	NAD-dependent,JW1375,htpH,hslI,hslF,Fermentative D-lactate dehydrogenase,ECK1377
Similar products	ldhA, D-lactate dehydrogenase, fermentative D-lactate dehydrogenase NAD-dependent, fermentative D-lactate dehydrogenase, NAD-dependent, ECK1377, hslF, hslI, htpH, JW1375, ATGP3236-10ug, ATGP3236-20ug, ATGP3236-50ug, ATGP3236-100ug, ATGP3236-250ug, ATGP3236-500ug, ATGP3236-1mg, ATGP3236-10, ATGP3236-20, ATGP3236-50, ATGP3236-100, ATGP3236-250, ATGP3236-500, ATGP3236-1
Available
Manufacturer - Type	Recombinant Protein
Manufacturer - Applications	SDS-PAGE, Enzyme Activity
Storage Conditions	Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Molecular Weight	39.1 kDa (353aa) confirmed by MALDI-TOF
Description	Idha, also known as D-lactate dehydrogenase, belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. In enzymology, a D-lactate dehydrogenase (cytochrome) is an enzyme that catalyzes the chemical reaction. Thus, the two substrates of this enzyme are (D) -lactate and ferricytochrome c, whereas its two products are pyruvate and ferrocytochrome c. Recombinant E. coli IdhA protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Concentration	1mg/ml (determined by Bradford assay)
Formulation	Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 100mM NaCl
Antigen Species	E.coli
Gene Category	Redox Proteins
Domain	1-329aa
Endotoxin	< 1 EU per 1ug of protein (determined by LAL method)
Bioactivity Comment Only	Specific activity is > 200unit/mg, in which one unit will convert 1.0 umole of pyruvate to L-lactate and beta-NAD per minute at pH 7.5 at 37C.
Species of Protein	E.coli
Manufacturer - Search Terms	D-lactate dehydrogenase, ldhA, fermentative D-lactate dehydrogenase NAD-dependent, fermentative D-lactate dehydrogenase, NAD-dependent, ECK1377, hslF, hslI, htpH, JW1375, ATGP3236-10ug, ATGP3236-20ug, ATGP3236-50ug, ATGP3236-100ug, ATGP3236-250ug, ATGP3236-500ug, ATGP3236-1mg, ATGP3236-10, ATGP3236-20, ATGP3236-50, ATGP3236-100, ATGP3236-250, ATGP3236-500, ATGP3236-1

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 100 ug

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Delivery expected until 7/31/2025

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