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Recombinant E.coli Beta-lactamase protein

Recombinant E.coli Beta-lactamase protein

Item no.	NKM-ATGP3575-500ug
Manufacturer	NKMAX
Amount	500 ug
Quantity options	100 ug 10 ug 20 ug 250 ug 500 ug 50 ug
Category	Food Safety Antibiotics Peptides & Proteins Recombinant Proteins SDS-PAGE
Type	Proteins Recombinant
Format	Liquid
Applications	SDS-PAGE
Specific against	Escherichia coli (E.coli)
Host	E.coli
Conjugate/Tag	HIS
Purity	> 95% by SDS-PAGE
Sequence	<MGSSHHHHHH SSGLVPRGSH M>APQQINDIV HRTITPLIEQ QKIPGMAVAV IYQGKPYYFT WGYADIAKKQ PVTQQTLFEL GSVSKTFTGV LGGDAIARGE IKLSDPTTKY WPELTAKQWN GITLLHLATY TAGGLPLQVP DEVKSSSDLL RFYQNWQPAW APGTQRLYAN SSIGLFGALA VKPSGLSFEQ AMQTRVFQPL KLNHTWINVP PAEEKNYAWG YREGKAVHVS
Citations	Zhao Z., et al. (2015) Cancer Lett. 358(1):17-26. Martin I., et al. (2014) Cell. 157(2):472-85.
NCBI	NP_418574
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	ampC,ampA,Cephalosporinase
Similar products	b-lactamase, ATGP3575-10ug, ATGP3575-20ug, ATGP3575-50ug, ATGP3575-100ug, ATGP3575-250ug, ATGP3575-500ug, ATGP3575-1mg, ATGP3575-10, ATGP3575-20, ATGP3575-50, ATGP3575-100, ATGP3575-250, ATGP3575-500, ATGP3575-1
Available
Manufacturer - Type	Recombinant Protein
Manufacturer - Applications	SDS-PAGE, Enzyme Activity
Storage Conditions	Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Molecular Weight	41.8 kDa (379aa) confirmed by MALDI-TOF
Description	AmpC, also known as Beta-lactamase, is the most widespread resistance mechanism to beta-lactam antibiotics, such as the penicillins and the cephalosporins. These antibiotics have a common element in their molecular structure: a four-atom ring known as a beta-lactam. The lactamase enzyme breaks that ring open, deactivating the molecule's antibacterial properties. Recombinant E. coli beta-lactamase protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
Concentration	1mg/ml (determined by Bradford assay)
Formulation	Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol
Antigen Species	E.coli
Gene Category	Metabolism
Domain	20-377aa
Bioactivity Comment Only	Specific activity is >700unit/mg, in which One unit will hydrolyze 1.0umole of Nitrocefin per minute at pH 7.0 at 37C
Species of Protein	E.coli
Manufacturer - Search Terms	b-lactamase, ATGP3575-10ug, ATGP3575-20ug, ATGP3575-50ug, ATGP3575-100ug, ATGP3575-250ug, ATGP3575-500ug, ATGP3575-1mg, ATGP3575-10, ATGP3575-20, ATGP3575-50, ATGP3575-100, ATGP3575-250, ATGP3575-500, ATGP3575-1

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 500 ug

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Delivery expected until 7/31/2025

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