Comparison

Recombinant SARS-CoV-2 (2019-nCoV) Spike S1 Subunit protein

Item no. NKM-ATGP3961-100ug
Manufacturer NKMAX
Amount 100 ug
Quantity options 100 ug 10 ug 20 ug 250 ug 500 ug 50 ug
Category
Type Proteins Recombinant
Format Liquid
Applications SDS-PAGE
Specific against SARS-CoV-2
Host HEK293
Conjugate/Tag HIS
Purity > 85% by SDS-PAGE
Sequence <DGSM>VNLTTR TQLPPAYTNS FTRGVYYPDK VFRSSVLHST QDLFLPFFSN VTWFHAIHVS GTNGTKRFDN PVLPFNDGVY FASTEKSNII RGWIFGTTLD SKTQSLLIVN NATNVVIKVC EFQFCNDPFL GVYYHKNNKS WMESEFRVYS SANNCTFEYV SQPFLMDLEG KQGNFKNLRE FVFKNIDGYF KIYSKHTPIN LVRDLPQGFS ALEPLVDLPI GINITRFQTL
Citations Wu, F. et al. (2020) Nature. 579:265-269.
Ortega, J.T. et al. (2020) EXCLI J. 19:410-417.
Wanbo T., et al, (2020) Cell. Mol. Immunol. 17:613-620.
Gorbalenya A., et al (2020) Nat. Microbiol. 5:536-544.
Tortorici, M.A. and D. Veesler (2019). Adv. Virus Res. 105:93-116.
NCBI QHD43416.1
ECLASS 10.1 32160409
ECLASS 11.0 32160409
UNSPSC 12352202
Alias Spike glycoprotein,S glycoprotein,E2,Peplomer protein,covid19,COVID-19,COVID-19 virus,HCoV-19,Human coronavirus 2019,SARS2,Spike protein S1,Severe acute respiratory syndrome coronavirus 2,2019-nCoV,S
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Available
Specificity SARS-CoV-2 (2019-nCoV)
Manufacturer - Type
Recombinant Protein
Manufacturer - Applications
SDS-PAGE, Bioactivity
Storage Conditions
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Molecular Weight
76.2kDa (680aa)
Description
An epidemic of acute respiratory syndrome in humans, which appeared in Wuhan, China in December 2019, was caused by a novel coronavirus (SARS-CoV-2). This disease was named as "Coronavirus Disease 2019" (COVID-19). This virus shares highly homological sequence with SARS-CoV, and causes acute, highly lethal pneumonia coronavirus disease 2019 (COVID-19) with clinical symptoms similar to those reported for SARS-CoV and MERS-CoV. The genome of this and other emerging pathogenic human CoVs encodes four major structural proteins [spike (S), envelope (E), membrane (M), and nucleocapsid (N)], approximately 16 nonstructural proteins (nsp1–16), and five to eight accessory proteins. Among them, the S protein plays an essential role in viral attachment, fusion, entry, and transmission. It comprises an N-terminal S1 subunit responsible for virus–receptor binding and a C-terminal S2 subunit responsible for virus–cell membrane fusion. S1 is further divided into an N-terminal domain (NTD) and a receptor-binding domain (RBD). SARS-CoV-2 and SARS-CoV bind angiotensin-converting enzyme 2 (ACE2) while MERS-CoV binds dipeptidyl peptidase 4 (DPP4), as receptors on the host cell expressing ACE2 (e.g., pneumocytes, enterocytes) or DPP4 (e.g., liver or lung cells including Huh-7, MRC-5, and Calu-3). During infection, CoV first binds the host cell through interaction between its S1-RBD and the cell membrane receptor, triggering conformational changes in the S2 subunit that result in virus fusion and entry into the target cell. Recombinant SARS-CoV-2 (2019-nCoV) Spike S1 Subunit, fused to His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.
Concentration
0.25mg/ml (determined by Absorbance at 280nm)
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Antigen Species
SARS-CoV-2 (2019-nCoV)
Gene Category
Virus
Domain
16-685aa
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Bioactivity Comment Only
Measured by its binding ability in a functional ELISA with Human ACE-2 (CAT# ATGP3963)
Predicted nterm
Asp
Species of Protein
SARS-CoV-2 (2019-nCoV)
Manufacturer - Search Terms
covid-19, covid19, sars, sars2, corona virus, ATGP3961-10ug, ATGP3961-20ug, ATGP3961-50ug, ATGP3961-100ug, ATGP3961-250ug, ATGP3961-500ug, ATGP3961-1mg, ATGP3961-10, ATGP3961-20, ATGP3961-50, ATGP3961-100, ATGP3961-250, ATGP3961-500, ATGP3961-1

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 100 ug
Available: In stock
available

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