Comparison

Recombinant human EGF protein

Item no. NKM-EGF0501-20ug
Manufacturer NKMAX
Amount 20 ug
Quantity options 100 ug 20 ug 250 ug 500 ug 50 ug
Category
Type Proteins Recombinant
Format Liquid
Applications SDS-PAGE
Specific against Human (Homo sapiens)
Host E.coli
Conjugate/Tag Untagged
Purity > 95% by SDS-PAGE
Sequence MNSDSECPLS HDGYCLHDGV CMYIEALDKY ACNCVVGYIG ERCQYRDLKW WELR
Citations Riese., et al (1998 ) Bioessays. 20: 41-48.
Cohen S., (1983) Cancer. 15: 1787-1791.
Carpenter G., et al (1979) Annu. Rev. Biochem., 48: 193-216.

Tazawa H, et al. Chronic inflammation-derived nitric oxide causes conversion of human colonic adenoma cells into adenocarcinoma cells. (Exp Cell Res. 2013){https://pubmed.ncbi.nlm.nih.gov/23948305/}
Park SY, et al. DNAJB1 negatively regulates MIG6 to promote epidermal growth factor receptor signaling. (Biochim Biophys Acta. 2015){https://pubmed.ncbi.nlm.nih.gov/26239118/}
Okuda K, et al. Pivotal role for S-nitrosylation of DNA methyltransferase 3B in epigenetic regulation of tumorigenesis. (Nat Commun. 2023){https://pubmed.ncbi.nlm.nih.gov/36739439/}
NCBI NP_001954.2
ECLASS 10.1 32160409
ECLASS 11.0 32160409
UNSPSC 12352202
Alias Epidermal growth factor,Pro-epidermal growth factor,urogastrone,EGF
Similar products EGF, epidermal growth factor (beta-urogastrone), EGF0501-10ug, EGF0501-20ug, EGF0501-50ug, EGF0501-100ug, EGF0501-250ug, EGF0501-500ug, EGF0501-1mg, EGF0501-10, EGF0501-20, EGF0501-50, EGF0501-100, EGF0501-250, EGF0501-500, EGF0501-1
Available
Manufacturer - Type
Recombinant Protein
Manufacturer - Applications
SDS-PAGE, Bioactivity
Storage Conditions
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Molecular Weight
6.3 kDa (54aa) confirmed by MALDI-TOF
Description
Recombinant human epidermal growth factor (EGF) is a 6. 3 kDa globular protein containing 54 amino acids residues, including 3 intra-molecular disulfide bonds. EGF is a potent growth factor that stimulates the proliferation of various epidermal and epithelial cells. Additionally, EGF has been shown to inhibit gastric secretion, and to be involved in wound healing. EGF signals through a receptor known as c-erbB, which is a class I tyrosine kinase receptor. Recombinant EGF was expressed in E. coli and purified by conventional column chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.
Concentration
1mg/ml (determined by Bradford assay)
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4)
Antigen Species
Human
Gene Category
Hormones & Cytokines
Domain
971-1023aa
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Bioactivity Comment Only
Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. The ED50 for this effect is less or equal to 0.1ng/ml.
Species of Protein
Human
Manufacturer - Search Terms
EGF, EGF0501-10ug, EGF0501-20ug, EGF0501-50ug, EGF0501-100ug, EGF0501-250ug, EGF0501-500ug, EGF0501-1mg, EGF0501-10, EGF0501-20, EGF0501-50, EGF0501-100, EGF0501-250, EGF0501-500, EGF0501-1, epidermal growth factor (beta-urogastrone)

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 20 ug
Available: In stock
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