Recombinant human PCNA protein

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

PCNA (Proliferating Cell Nuclear Antigen) is found in the nucleus and is a cofactor of DNA polymerase delta. This protein is associated with DNA synthesis and repair. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. It appears during late G1- phase, S-phase of mitosis and persists until the end of the M-phase because of its long biological half-life. PCNA may be induced by uV irradiation, growth factors and eventually by neighbouring tumours. Recombinant human PCNA, was expressed in E. coli and purified by conventional chromatography techniques.

Liquid in. 20mM Tris-HCl buffer (pH 7.5) containing 2mM EDTA, 20% glycerol

Cell Cycle

Human