Recombinant human PRMT1 protein

SDS-PAGE, Enzyme Activity

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Protein arginine N-methyltransferase 1 (PRMT1) is a type I methyltransferase that transfers a methyl group from S-adenosylmethionine to guanidino nitrogens of arginine residues to form monomethylarginine and asymmetric dimethylarginine. Functions of type I arginine methylation in proteins may include regulation of transcription, modulation of the affinity of nucleic acid-binding proteins, regulation of interferon signaling pathways, and targeting of nuclear proteins. Recombinant human PRMT1, fused to His-MBP tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Liquid in. 40mM Tris-HCl buffer (pH 8.0) containing 100mM NaCl, 4mM MgCl₂, 2mM DTT, 40% glycerol

Histone Related

Specific activity is > 10 nmol/min/mg, and is defined as the amount of enzyme that transfer 1.0 nmole of methyl group on histone H4 per minute at 37C.

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